Effect of compressed fluids treatment on β-galactosidase activity and stability

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• 作者：Anna Rafaela Cavalcante Braga (1) annabraga@furg.br
  Marceli Fernandes Silva (2)
  J. Vladimir Oliveira (3) vladimir@uri.com.br
  Helen Treichel (14)
  Susana Juliano Kalil (1) dqmsjk@furg.br
• 关键词：Carbon dioxide – Kluyveromyces marxianus – n ; Butane – Propane – LPG
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2012
• 出版时间：November 2012
• 年：2012
• 卷：35
• 期：9
• 页码：1541-1547
• 全文大小：256.2 KB
• 参考文献：1. Gekas V, López-Leiva M (1985) Hydrolysis of lactose: a literature review. Process Biochem 20:1–12
  2. Ustok FI, Tari C, Harsa S (2010) Biochemical and thermal properties of [beta]-galactosidase enzymes produced by artisanal yoghurt cultures. Food Chem 119:1114–1120
  3. Hsu CA, Yu RC, Lee SL, Chou CC (2007) Cultural condition affecting the growth and production of beta-galactosidase by Bifidobacterium longum CCRC 15708 in ajar fermenter. Int J Food Micobiol 116:186–189
  4. Ladero M, Santos A, Garcia-Ochoa F (2000) Kinetic modeling of lactose hydrolysis with an immobilized [beta]-galactosidase from Kluyveromyces fragilis. Enzyme Microb Technol 27:583–592
  5. Jurado E, Camacho F, Luzon G, Vicaria JM (2004) Kinetic models of activity for [beta]-galactosidases: influence of pH, ionic concentration and temperature. Enzyme Microb Technol 34:33–40
  6. Kuhn GO, Coghetto C, Treichel H, de Oliveira D, Oliveira JV (2011) Effect of compressed fluids treatment on the activity of inulinase from Kluyveromyces marxianus NRRL Y-7571 immobilized in montmorillonite. Process Biochem (in press)
  7. Oliveira D, Feihrmann AC, Dariva C, Cunha AG, Bevilaqua JV, Destain J, Oliveira JV, Freire D (2006) Influence of compressed fluids treatment on the activity of Yarrowia lipolytica lipase. J Mol Catal Enzym 39:117–123
  8. Ceni G, da Silva PC, Lerin L, Charin RM, Oliveira JV, Toniazzo G, Treichel H, Oestreicher EG, de Oliveira D (2010) Enzyme-catalyzed production of 1-glyceryl benzoate in compressed n-butane. Enzyme Microb Technol 46:513–519
  9. Knez Z, Habulin M (2002) Compressed gases as alternative enzymatic-reaction solvents: a short review. J Supercrit Fluids 23:29–42
  10. Oliveira D, Feihrmann AC, Rubira AF, Kunita MH, Dariva C, Oliveira JV (2006) Assessment of two immobilized lipases activity treated in compressed fluids. J Supercrit Fluids 38:373–382
  11. Kuhn G, Marangoni M, Freire DMG, Soares VrF, de Godoy MG, de Castro AM, Luccio MD, Treichel H, Mazutti MA, Oliveira D, Oliveira JV (2010) Esterification activities of non-commercial lipases after pre-treatment in pressurized propane. J Chem Technol Biotechnol 85:839–844
  12. Manera AP, Kuhn G, Polloni A, Marangoni M, Zabot G, Kalil SJ, Oliveira Dd, Treichel H, Vladimir Oliveira J, Mazutti MA, Maugeri F (2011) Effect of compressed fluids treatment on the activity, stability and enzymatic reaction performance of [beta]-galactosidase. Food Chem 125:1235–1240
  13. Manera AP, Ores JC, Ribeiro VA, Burkert CAV, Kalil SJ (2008) Optimization of the culture medium for the production of β-galactosidase from Kluyveromyces marxianus CCT 7082. Food Technol Biotechnol 46:66–72
  14. Pinheiro R, Belo I, Mota M (2003) Growth and β-galactosidase activity in cultures of Kluyveromyces marxianus under increased air pressure. Lett Appl Microbiol 37:438–442
  15. Lukondeh T, Ashbolt NJ, Rogers PL (2005) Fed-batch fermentation for production of Kluyveromyces marxianus FII 510700 cultivated on a lactose-based medium. J Ind Microbiol Biotechnol 32:284–288
  16. Medeiros FO, Alves FG, Lisboa CR, de Souza Martins D, Burkert CAV, Kalil SJ (2008) Ultrasonic waves and glass pearls: a new method of extraction of beta-galactosidase for use in laboratory. Quim Nova 31:336–339
  17. Fricks AT, Souza DPB, Oestreicher EG, Antunes OAC, Girardi JS, Oliveira D, Dariva C (2006) Evaluation of radish (Raphanus sativus L.) peroxidase activity after high-pressure treatment with carbon dioxide. J Supercrit Fluids 38:347–353
  18. Inchaurrondo VA, Yautorno OM, Voget CE (1994) Yeast growth and β-galactosidase production during aerobic batch cultures in lactose-limited synthetic medium. Process Biochem 29:47–54
  19. Oliveira D, Feihrmann AC, Dariva C, Cunha AG, Bevilaqua JV, Destain J, Oliveira JV, Freire DMG (2006) Influence of compressed fluids treatment on the activity of Yarrowia lipolytica lipase. J Mol Catal Enzym 39:117–123
  20. Dwevedi A, Kayastha AM (2009) A beta-galactosidase from pea seeds (PsBGAL): purification, stabilization, catalytic energetics, conformational heterogeneity, and its significance. J Agric Food Chem 57:7086–7096
  21. Zhou QZK, Chen XD (2001) Effects of temperature and pH on the catalytic activity of the immobilized galactosidase from Kluyveromyces lactis. Biochem Eng J 9:33–44
  22. Nagy Z, Kiss T, Szentirmai A, Biro S (2001) Beta-galactosidase of Penicillium chrysogenum: production, purification, and characterization of the enzyme. Protein Expr Purif 21:24–29
• 作者单位：1. Chemistry and Food School, Federal University of Rio Grande-FURG, P.O. Box 474, Rio Grande, RS CEP 96201900, Brazil2. Department of Food Engineering, URI-Campus de Erechim, P.O. Box 743, Erechim, RS CEP 99700-000, Brazil3. Department of Chemistry and Food Engineering, Federal University of Santa Catarina, UFSC, Campus Universitário, Bairro Trindade, P.O. Box 476, Florianópolis, SC CEP 88040-900, Brazil4. Federal University of Fronteira Sul, Campus de Erechim, Av. Dom Jo?o Hoffmann, 313, Erechim, RS CEP 99700-000, Brazil
• ISSN：1615-7605

文摘

This study aimed at assessing the influence of different pressurized fluids treatment on the enzymatic activity and stability of a lyophilized β-galactosidase. The effects of system pressure, exposure time and depressurization rate, using propane, n-butane, carbon dioxide and liquefied petroleum gas on the enzymatic activity were evaluated. The β-galactosidase activity changed significantly depending on the experimental conditions investigated, allowing the selection of the proper compressed fluid for advantageous application of this biocatalyst in enzymatic reactions. The residual activity ranged from 32.1 to 93.8 % after treatment. The storage stability of the enzyme after high-pressure pre-treatment was also monitored, and results showed that the biocatalyst activity presents strong dependence of the fluid used in the pretreatment. The activity gradually decreases over the time for the enzyme treated with LGP and propane, while the enzyme treated with n-butane maintained 96 % of its initial activity until 120 days. For CO₂, there was a reduction of around 40 % in the initial activity 90 days of storage. The enzyme treated with n-butane also showed a better thermostability in terms of enzymatic half-life.