Single Strand DNA Breaks in Human Lymphocytes Exposed to para -Phenylenediamine and its Derivatives

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• 作者：Soi Mei Chye ; You Cheng Hseu ; Shih-Hsiung Liang ; Chin-Hui Chen ; Ssu Ching Chen
• 关键词：para ; Phenylenediamine (CAS 106 ; 50 ; 3) ; Comet assay ; Lymphocytes ; Genotoxicity
• 刊名：Bulletin of Environmental Contamination and Toxicology
• 出版年：2008
• 出版时间：January, 2008
• 年：2008
• 卷：80
• 期：1
• 页码：58-62
• 全文大小：251.4 KB

文摘

Para-Phenylenediamine (PPD), the main aromatic amines used in the hair dye formation, and its four derivatives (2-chloro-p-phenylenediamine, 4-chloro-o-phenylenediamine, 2-nitro-p-phenylenediamine, and 4-nitro-o-phenylenediamine) were examined for their potential to produce single strand DNA breaks in human lymphocytes using the alkaline comet assay. Results revealed that all the tested chemicals within the range of doses from 100 μM to 500 μM showed the genotoxicity in a dose-dependent manner after the incubation of lymphocytes with these chemicals for 2 h. In this study, we first reported that PPD and its four derivatives can elicit the type of single strand breaks in human lymphocytes.