Cardiac connexins Cx43 and Cx45: formation of diverse gap junction channels with diverse electrical properties

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• 作者：Thomas Desplantez (1)
  Deborah Halliday (2)
  Emmanuel Dupont (2)
  Robert Weingart (1)
  
• 关键词：Connexin43 ; Connexin45 ; Heterotypic channels ; Heteromeric channels ; Electrical properties
• 刊名：Pfl眉gers Archiv - European Journal of Physiology
• 出版年：2004
• 出版时间：July 2004
• 年：2004
• 卷：448
• 期：4
• 页码：363-375
• 全文大小：489KB
• 参考文献：1. Bader P, Weingart R (2003) Conductive and kinetic properties of connexin45 hemichannels (abstract). Pflugers Arch 445:R69
  2. Banach K, Weingart R (2000) Voltage gating of Cx43 gap junction channels involves fast and slow current transitions. Pflugers Arch 439:248鈥?50 CrossRef
  3. Barrio LC, Capel J, Jarillo JA, Castro C, Revilla A (1997) Species-specific voltage-gating properties of connexin-45 junctions expressed in Xenopus oocytes. Biophys J 73:757鈥?69
  4. Brink PR, Cronin K, Banach K, Peterson E, Westphale EM, Seul KH, Ramanan SV, Beyer EC (1997) Evidence for heteromeric gap junction channels formed from rat connexin43 and human connexin37. Am J Physiol 273:C1386鈥揅1396
  5. Bruzzone R, White WT, Paul DL (1996) Connection with connexins: the molecular basis of direct intercellular signaling. Eur J Biochem 138:1鈥?7
  6. Chen-Izu Y, Moreno AP, Spangler RA (2001) Opposing gates model for voltage gating of gap junction channels. Am J Physiol 281:C1604鈥揅1613
  7. Contreras JE, Saez JC, Bukauskas FF, Bennett MVL (2003) Gating and regulation of connexin 43 (Cx43) hemichannels. Proc Natl Acad Sci USA 100:11388鈥?1393 CrossRef
  8. Coppen SR, Kodama I, Boyett MR, Dobrzynski H, Takagishi Y, Honjo H, Yeh HI, Severs NJ (1999) Connexin45, a major connexin of the rabbit sinoatrial node, is co-expressed with connexin43 in a restricted zone at the nodal-crista terminalis border. J Histochem Cytochem 47:907鈥?18
  9. Cottrell GT, Wu Y, Burt JM (2002) Cx40 and Cx43 expression ratio influences heteromeric/heterotypic gap junction channel properties. Am J Physiol 282:C1469鈥揅1482
  10. Davis LM, Rodefeld ME, Green K, Beyer EC, Saffitz JE (1995) Gap junction protein phenotypes of the human heart and conduction system. J Cardiovasc Electrophysiol 6:813鈥?22
  11. Desplantez T, Weingart R (2003) Connexins Cx43 and Cx45: diversity of channels鈥攄iversity of electrical properties. In: Proceedings of the 2003 International Gap Junction Conference, Cambridge, UK, August 23鈥?8, p 57
  12. Elenes S, Martinez AD, Delmar M, Beyer EC, Moreno AP (2001) Heterotypic docking of Cx43 and Cx45 connexons blocks fast voltage gating of Cx43. Biophys J 81:1406鈥?418
  13. Gros DB, Jongsma HJ (1996) Connexins in mammalian heart function. Bioessays 18:719鈥?30
  14. Halliday D, Dupont E, Coppen SR, Severs NJ (2003) Development of a cell model for functional and structural analysis of connexin co-expression: achieving homogeneous and inducible expression of multiple connexins in stable transfectants. Cell Commun Adhes 10:1鈥? CrossRef
  15. Harris AL (2001) Emerging issues of connexin channels: biophysics fills the gap. Q Rev Biophys 34:325鈥?72
  16. He DS, Jiang JX, Taffet SM, Burt JM (1999) Formation of heteromeric gap junction channels by connexins 40 and 43 in vascular smooth muscle cells. Proc Natl Acad Sci USA 96:6495鈥?500 CrossRef
  17. Kanter HL, Laing JG, Beau SL, Beyer EC, Saffitz JE (1993) Distinct patterns of connexin expression in canine Purkinje fibers and ventricular muscle. Circ Res 72:1124鈥?131
  18. Kanter HL, Laing JG, Beyer EC, Green KG, Saffitz JE (1993) Multiple connexins colocalize in canine ventricular myocyte gap junctions. Circ Res 73:344鈥?50
  19. Kistler J, Lin JS, Bond J, Green C, Eckert R, Merriman R, Tunstall M, Donaldson P (1999) Connexins in the lens: are they to blame in diabetic cataractogenesis? In: Gap junction-mediated intercellular signalling in health and disease (Novartis Foundation Symposium series, Vol. 219). Wiley, Chichester, pp 97鈥?08
  20. Kl茅ber AG, Rudy Y (2004) Basic mechanisms of cardiac impulse propagation and associated arrhythmias. Physiol Rev 84:431鈥?88
  21. Lee MJ, Rhee SK (1998) Heteromeric gap junction channels in rat hepatocytes in which the expression of connexin26 is induced. Mol Cells 8:295鈥?00
  22. Moreno AP, Fishman GI, Beyer EC, Spray DC (1995) Voltage dependent gating and single channels analysis of heterotypic gap junction channels formed of Cx45 and Cx43. Progr Cell Res 4:405鈥?08
  23. Polontchouk, LD, Valiunas V, Haefliger J-A, Eppenberger HM, Weingart R (2002) Expression and regulation of connexins in cultured ventricular myocytes isolated from adult rat hearts. Pflugers Arch 443:676鈥?89
  24. Severs NJ (1999) Cardiovascular disease. In: Gap Junction-mediated intercellular signalling in health and disease (Novartis Foundation Symposium series, Vol. 219). Wiley, Chichester, pp 188鈥?10
  25. Steiner E, Ebihara L (1996) Functional characterization of canine connexin45. J Membr Biol 150:153鈥?61 CrossRef
  26. Trexler EB, Bennett, MVL, Bargiello TA, Verselis VK (1996) Voltage gating and permeation in a gap junction hemichannel. Proc Natl Acad Sci USA 93:5836鈥?841 CrossRef
  27. Valiunas V (2002) Biophysical properties of connexin-45 gap junction hemichannels studied in vertebrate cells. J Gen Physiol 119:147鈥?64 CrossRef
  28. Valiunas V, Bukauskas FF, Weingart R (1997) Conductances and selective permeability of connexin43 gap junction channels examined in neonatal rat heart cells. Circ Res 80:708鈥?19
  29. Valiunas V, Manthey D, Vogel R, Willecke K, Weingart R (1999) Biophysical properties of mouse connexin30 gap junction channels studied in transfected human HeLa cells. J Physiol (Lond) 519:631鈥?44
  30. Valiunas V, Weingart R (2000) Electrical properties of gap junction hemichannels identified in transfected HeLa cells. Pflugers Arch 440:366鈥?79 CrossRef
  31. Valiunas V, Weingart R, Brink PR (2000) Formation of heterotypic gap junction channels by connexins 40 and 43. Circ Res 86:E42鈥揈49
  32. Valiunas V, Gemel J, Brink PR, Beyer EC (2001) Gap junction channels formed by coexpressed connexin40 and connexin43. Am J Physiol 281: H1675鈥揌1689
  33. Van Rijen HVM, Wilders R, Van Ginneken ACG, Jongsma HJ (1998) Quantitative analysis of dual whole-cell voltage-clamp determination of gap junctional conductance. Pflugers Arch 436:141鈥?51
  34. Van Veen AA, van Rijen HV, Opthof T (2001) Cardiac gap junction channels: modulation of expression and channel properties. Cardiovasc Res 51:217鈥?29 CrossRef
  35. Vogel R, Weingart R (1998) Mathematical model of vertebrate gap junctions derived from electrical measurements on homotypic and heterotypic channels. J Physiol (Lond) 510:177鈥?89
  36. Wang XG, Peracchia C (1998) Chemical gating of heteromeric and heterotypic gap junction channels. J Membr Biol 1998 162:169鈥?76 CrossRef
  37. Willecke K, Eiberger J, Degen J, Eckardt D, Romualdi A, Guldenagel M, Deutsch U, Sohl G (2002) Structural and functional diversity of connexin genes in the mouse and human genome. Biol Chem 383:725鈥?37
  
• 作者单位：Thomas Desplantez (1)
  Deborah Halliday (2)
  Emmanuel Dupont (2)
  Robert Weingart (1)
  
  1. Physiologisches Institut, Universit盲t Bern, B眉hlplatz 5, 3012, Bern, Switzerland
  2. National Heart and Lung Institute (Imperial College London), Royal Brompton Hospital, Sydney Street, London, SW3 6NP, UK
  
• ISSN：1432-2013

文摘

HeLa cells expressing rat connexin43 (Cx43) and/or mouse Cx45 were studied with the dual voltage-clamp technique. Different types of cell pairs were established and their gap junction properties determined, i.e. the dependence of the instantaneous and steady-state conductances (g j,inst, g j,ss) on the transjunctional voltage (V j) and the kinetics of inactivation of the gap junction current (I j). Pairs of singly transfected cells showed homogeneous behaviour at both V j polarities. Homotypic Cx43-Cx43 and Cx45-Cx45 cell pairs yielded distinct symmetrical functions g j,inst=f(V j) and g j,ss=f(V j). Heterotypic Cx43-Cx45 preparations exhibited asymmetric functions g j,inst=f(V j) and g j,ss=f(V j) suggesting that connexons Cx43 and Cx45 gate with positive and negative V j, respectively. Preparations containing a singly (Cx43 or Cx45) or doubly (Cx43/45) transfected cell showed quasi-homogeneous behaviour at one V j polarity and heterogeneous behaviour at the other polarity. The former yielded Boltzmann parameters intermediate between those of Cx43-Cx43, Cx45-Cx45 and Cx43-Cx45 preparations; the latter could not be explained by homotypic and heterotypic combinations of homomeric connexons. Each pair of doubly transfected cells (Cx43/Cx45) yielded unique functions g j,inst=f(V j) and g j,ss=f(V j). This can not be explained by combinations of homomeric connexons. We conclude that Cx43 and Cx45 form homomeric-homotypic, homomeric-heterotypic channels as well as heteromeric-homotypic and heteromeric-heterotypic channels. This has implications for the impulse propagation in specific areas of the heart.