Highly selective determination of cysteine using a composite prepared from multiwalled carbon nanotubes and gold nanoparticles stabilized with calcium crosslinked pectin

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• 作者：Rajkumar Devasenathipathy ; Chelladurai Karuppiah ; Shen-Ming Chen…
• 关键词：Multiwalled carbon nanotubes ; Pectin ; Electrodeposition ; Gold nanoparticles ; L ; Cysteine ; Amperometry
• 刊名：Microchimica Acta
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：182
• 期：3-4
• 页码：727-735
• 全文大小：1,383 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073

文摘

We describe a glassy carbon electrode (GCE) modified with gold nanoparticles that were stabilized with calcium-crosslinked pectin (CCLP) and electrodeposited on multiwalled carbon nanotubes (MWCNTs) by cyclic voltammetry. The resulting electrode was used for the selective determination of L-cysteine (L-Cys). Its characterization showed that the CCLP acts as a scaffold to form highly stable, uniform and electrochemically active AuNPs. Electrochemical studies showed the MWCNT to significantly promote the electrodeposition of the CCLP-AuNPs. The new GCE exhibited excellent electrocatalytic ability towards oxidation of L-Cys in showing a lower overpotential and giving a higher oxidation peak current. The diffusion coefficient for the oxidation of L-Cys was calculated to be 3.0 × 10? cm2 s?. This amperometric sensor displays a wide linear range (from 0.1 to 1,000 μM), high sensitivity (0.46 μA μM? cm?) and a detection limit as low as 19 nM (at a signal-to-noise ratio of 3). The sensor was applied to specifically detect L-Cys even in the presence of 500-fold excess of interferents. It also is stable and possesses good repeatability and reproducibity, and was successfully applied to the determination of L-Cys in spiked samples of human serum. Graphical abstract Calcium ions cross linked pectin (CCLP) stabilized gold nanoparticles (AuNPs) at multiwalled carbon nanotubes (MWCNT) was electrochemically prepared. The as-prepared nanocomposite was characterized by various methods and employed for the selective determination of L-Cysteine.