Enzymatic glucose biosensor based on bismuth nanoribbons electrochemically deposited on?reduced graphene oxide

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• 作者：Rajkumar Devasenathipathy ; Raj Karthik ; Shen-Ming Chen…
• 关键词：Potentiostatic?method ; Bismuth nanoribbons ; Glucose oxidase ; Biosensor ; Reduced graphene oxide
• 刊名：Microchimica Acta
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：182
• 期：13-14
• 页码：2165-2172
• 全文大小：1,353 KB
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• 作者单位：Rajkumar Devasenathipathy (1)
  Raj Karthik (1)
  Shen-Ming Chen (1)
  Mohammad Ajmal Ali (2)
  Veerappan Mani (1)
  Bih-Show Lou (3)
  Fahad Mohammed Abdullrahman Al-Hemaid (2)
  
  1. Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei, 106, Taiwan, Republic of China
  2. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia
  3. Chemistry Division, Center for General Education, Chang Gung University, Tao-Yuan, 333, Taiwan
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073

文摘

We describe the electrochemical preparation of bismuth nanoribbons (Bi-NRs) with an average length of 100?±-0 nm and a width of 10?±- μm by a potentiostatic method. The process occurs on the surface of a glassy carbon electrode (GCE) in the presence of disodium ethylene diamine tetraacetate that acts as a scaffold for the growth of the Bi-NRs and also renders them more stable. The method was applied to the preparation of Bi-NRs incorporated into reduced graphene oxide. This nanocomposite was loaded with the enzyme glucose oxidase onto a glassy carbon electrode. The resulting biosensor displays an enhanced redox peak for the enzyme with a peak-to-peak separation of about 28 mV, revealing a fast electron transfer at the modified electrode. The loading of the GCE with electroactive GOx was calculated to be 8.54?×-0?0 mol?cm?, and the electron transfer rate constant is 4.40 s?. Glucose can be determined (in the presence of oxygen) at a relatively working potential of ?.46 V (vs. Ag|AgCl) in the 0.5 to 6 mM concentration range, with a 104 μM lower detection limit. The sensor also displays appreciable repeatability, reproducibility and remarkable stability. It was successfully applied to the determination of glucose in human serum samples.