Development and characterization of microsatellite loci for Rhinogobius sp. YB (Gobiidae)

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• 作者：Noriyuki Koizumi (1)
  Takumi Moriyama (2)
  Tetsuo Yoshino (3)
  Kazuya Nishida (4)
  Atsushi Mori (1)
  Keiji Watabe (1)
  Takeshi Takemura (1)
  
  1. National Institute for Rural Engineering ; Tsukuba ; Ibaraki ; 305-8609 ; Japan
  2. Faculty of Agriculture ; Utsunomiya University ; Utsunomioya ; Tochigi ; 321-8505 ; Japan
  3. General Research Center ; Okinawa Churashima Foundation ; Kunigami ; Okinawa ; 905-0206 ; Japan
  4. National Research Institute of Far Seas Fisheries ; Kanazawa ; Yokohama ; Kanagawa ; 236-8648 ; Japan
  
• 关键词：Gobiidae ; Microsatellite ; Genetic diversity ; Ryukyu Archipelago
• 刊名：Conservation Genetics Resources
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：7
• 期：1
• 页码：137-141
• 全文大小：159 KB
• 参考文献：1. Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202鈥?22 CrossRef
  2. Kano Y, Nishida S, Nakajima J (2012) Waterfalls drive parallel evolution in a freshwater goby. Ecol Evol 2:1805鈥?817 CrossRef
  3. Kon T, Yoshino T (2003) Coloration and ontogenetic features of fluviatile species of / Rhinogobius (Gobioidei: Gobiidae) in Amami-oshima Island, Ryukyu Islands, Japan. Ichthyol Res 50:109鈥?16 CrossRef
  4. Ohara K, Takahashi D, Takagi M (2004) Isolation of microsatellite loci in the freshwater goby, / Rhinogobius sp. (Gobiidae). Mol Ecol Notes 4:449鈥?51 CrossRef
  5. Ohara K, Takagi M, Hashimoto M, Miyazaki K, Hirashima K (2008) DNA markers indicate low genetic diversity and high genetic divergence in the landlocked freshwater goby, / Rhinogobius sp. YB, in the Ryukyu Archipelago. Japan. Zool sci 25:391鈥?00 CrossRef
  
• 刊物主题：Conservation Biology/Ecology; Plant Sciences; Animal Anatomy / Morphology / Histology; Evolutionary Biology;
• 出版者：Springer Netherlands
• ISSN：1877-7260

文摘

Rhinogobius sp. YB (Gobiidae) distributed in the Ryukyu Archipelago is a fluvial species of gobies. We present 34 novel microsatellite loci that were tested on 32 individuals from the Okierabu-jima Island in Kagoshima Prefecture. Seventeen loci were polymorphic, while the remaining 17 were monomorphic. The number of alleles per the polymorphic loci ranged from 2 to 15. The observed heterozygosities ranged from 0.038 to 1.000 and the expected heterozygosities varied from 0.038 to 0.816. These microsatellite loci will enhance future investigations on the genetic differentiation and structure of populations of R. sp. YB.