Homocitrate Synthase Expression and Lysine Content in Fruiting Body of Different Developmental Stages in Flammulina velutipes

详细信息    查看全文

• 作者：Fang Liu ; Wei Wang ; Bing-Zhi Chen ; Bao-Gui Xie
• 刊名：Current Microbiology
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：70
• 期：6
• 页码：821-828
• 全文大小：1,192 KB
• 参考文献：1.Altschul S, Madden T, Sch?ffer A, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389View Article PubMed Central PubMed
  2.Bailey T, Williams N, Misleh C, Li WW (2006) MEME: discovering and analyzing DNA and protein sequence motifs. Nucleic Acids Res 34:369-73View Article
  3.Bhattacharjee JK (1985) α-Aminoadipate pathway for the biosynthesis of lysine in lower eukaryotes. Crit Rev Microbiol 12:131-51View Article PubMed
  4.Bhattacharjee JK (1992) Evolution of α-aminoadipate pathway for the synthesis of lysine in fungi. In: Mortlock RP (ed) Handbook of evolution of metabolic function. CRC Press, Boca Raton, pp 47-0
  5.Cai HH, Liu XM, Chen ZY (2013) Isolation, purification and identification of nine chemical compounds from Flammulina velutipes fruiting bodies. Food Chem 141:2873-879View Article PubMed
  6.Cong WR, Liu Y, Li QZ, Zhou XW (2014) Cloning and analysis of a functional promoter of fungal immunomodulatory protein from Flammulina velutipes. Mol Biol Rep 41:4381-387View Article PubMed
  7.Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-91View Article
  8.Fukushima M, Ohashi T, Fujiwara Y, Sonoyama K, Nakano M (2001) Cholesterol-lowering effects of maitake (Grifola frondosa) fiber, shiitake (Lentinus edodes) fiber, and enokitake (Flammulina velutipes) fiber in rats. Exp Biol Med 226:758-65
  9.Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins M, Appel R, Bairoch A (2005) Protein identification and analysis tools on the ExPASy server. In: John MW (ed) The proteomics protocols handbook. Humana Press, Totowa, pp 571-07View Article
  10.Geourjon C, Deléage G (1995) SOPMA: significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments. Comput Appl Biosci 11:681-84PubMed
  11.Grossetête S, Labedan B, Lespinet O (2010) FUNGIpath: a tool to assess fungal metabolic pathways predicted by orthology. BMC Genom 11:81View Article
  12.Gruen HE, Wong WM (1982) Distribution of cellular amino acids, protein, and total organic nitrogen during fruitbody development in Flammulina velutipes growth on sawdust medium. Can J Bot 60:1330-341View Article
  13.Jiang JH, Liu F, Xu M (2006) Research of transforming lysine-rich protein gene to rice. J Fujian Agric For Univ 6:615-18
  14.Kim H, Lee W, Park YJ, Kim H, Beak J, Chung I, Kim JK, Lee J, Kang E, Kim S (2010) Whole genome sequencing of the winter mushroom, Flammulina velutipes. Plant Anim Genomes 17:9-3
  15.Kumar S, Tamura K, Jakobsen I, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244-245View Article PubMed
  16.Leifa F, Pandey A, Soccol CR (2001) Production of Flammulina velutipes on coffee husk and coffee spent-ground. Braz Arch Biol Technol 44:205-12View Article
  17.Luo RB, Liu BH, Xie YL, Li ZY, Huang WH, Yuan JY, He GZ, Chen YX, Pan Q, Liu YJ, Tang JB, Wu GX, Zhang H, Shi YJ, Liu Y, Yu C, Wang B, Lu Y, Han CL, Cheung DW, Yiu SM, Liu GM, Xiaoqian Z, Peng SL, Li YR, Yang HM, Wang J, Lam TW, Wang J (2012) SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. GigaScience 1:18View Article PubMed Central PubMed
  18.Mortazavi A, Williams BA, McCue K, Schaeffer L (2008) Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods 5:621-28View Article PubMed
  19.Nei M, Kumar S (2000) Phylogenetic trees. Molecular evolution and phylogenetics. OUP, New York, pp 73-6
  20.Quevillon E, Silventoinen V, Pillai S, Harte N, Mulder N, Apweiler R, Lopez R (2005) InterProScan: protein domains identifier. Nucleic Acids Res 33:116-20View Article
  21.Rodgers JL, Nicewander WA (1988) Thirteen ways to look at the correlation coefficient. Am Stat 42:59-6View Article
  22.Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406-25PubMed
  23.Smiderlea FR, Carboneroa ER, Sassakia GL, Gorina PAJ, Iacomini M (2008) Characterization of a heterogalactan: some nutritional values of the edible mushroom Flammulina velutipes. Food Chem 108:329-33View Article
  24.Stajich JE, Wilke SK, Ahrén D, Au CH, Birren BW, Borodovsky M, Burns C, Casselton LA, Cheng CK, Deng JX, Dietrich FS, Fargo DC, Farman ML, Gathman AC, Goldberg J, Guigó R, Hoegger PJ, Hooker JB, Huggins A, James TY, Kamada T, Kilaru S, Kodira C, Kües U, Kupfer D, Kwan HS, Lomsadze A (2010) Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus). Proc Natl Acad Sci USA 107:11889-1894View Article PubMed Central PubMed
  25.Stanke M, Diekhans M, Baertsch R, Haussler D (2008) Using native and syntenically mapped cDNA alignments to improve de novo gene finding. Bioinformatics 24:637-44View Article PubMed
  26.Stigler SM (1989) Franci
• 作者单位：Fang Liu (1)
  Wei Wang (1)
  Bing-Zhi Chen (1)
  Bao-Gui Xie (1)
  
  1. Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  
• 出版者：Springer New York
• ISSN：1432-0991

文摘

Homocitrate synthase (EC 2.3.3.14) regulates the first step of fungal lysine biosynthesis. The gene encoding homocitrate synthase was identified in whole genomic sequencing of Flammulina velutipes and contains seven introns. The homocitrate synthase gene of F. velutipes strain W23 (Fvhcs) is 1780?bp in length and encodes a 464 amino acid protein with a predicted molecular weight 50.7?kDa. Phylogenetic analysis of Fvhcs and other homocitrate synthase proteins from diverse fungi produced a topology congruent with the current best estimate of organismal phylogeny. Analysis of protein domains by InterProScan and a motif search found that Fvhcs gene encodes homocitrate synthase protein conserved across Agaricomycotina. In addition, we sequenced the transcriptome of different developmental stages and structures of the fruiting body to analyze the expression levels of the Fvhcs gene. The data showed a correlation between Fvhcs gene expression and lysine values in different developmental stages and structures of F. velutipes.