Activity of DNA polymerase λ in spikelets of rice and maize

详细信息    查看全文

• 作者：S. Bakshi ; S. Sihi ; D. N. Sengupta
• 关键词：Additional key wordsDNA repair ; in ; gel activity assay ; meiosis ; Oryza sativa ; Zea mays
• 刊名：Biologia Plantarum
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：60
• 期：4
• 页码：788-792
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Sciences;
• 出版者：Springer Netherlands
• ISSN：1573-8264
• 卷排序：60

文摘

Involvement of DNA polymerase (pol) enzymes in meiotic DNA repair has not been clearly understood in plants. DNA polymerase λ is involved in short patch DNA synthesis and base excision repair in both plants and animals. The presence and activity of the pol λ enzyme was studied in a protein isolated from spikelets during flower development stages of rice (Oryza sativa L.) and maize (Zea mays L.). Western blot analysis showed a 2- to 2.5-fold higher accumulation of pol λ in spikelets than in shoots. Assays of pol and in-gel activity showed the dideoxynucleotide triphosphate sensitive pol λ enzyme in spikelets of both the plants. An enhanced presence of the enzyme and its high activity suggests an active role of pol λ in meiotic recombination during microspore development.