Mycotoxin production of Fusarium langsethiae and Fusarium sporotrichioides on cereal-based substrates

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• 作者：M. Kokkonen (1)
  M. Jestoi (1)
  A. Laitila (2)
  
• 关键词：Cereal ; Fusarium ; LC ; MS/MS ; Mycotoxin production ; Substrate
• 刊名：Mycotoxin Research
• 出版年：2012
• 出版时间：February 2012
• 年：2012
• 卷：28
• 期：1
• 页码：25-35
• 全文大小：349KB
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• 作者单位：M. Kokkonen (1)
  M. Jestoi (1)
  A. Laitila (2)
  
  1. Finnish Food Safety Authority (Evira), Mustialankatu 3, 00790, Helsinki, Finland
  2. VTT Technical Research Centre of Finland, P.O. Box 1000, 02044, VTT, Finland
  

文摘

The present study investigated and compared the mycotoxin production of two Fusarium species, F. sporotrichioides and F. langsethiae, isolated from grain samples. Fusarium strains were cultivated at 25°C for 7 days on two types of solid media, i.e. rice-flour and cereal-flour agar. Toxins produced were measured after the incubation period with a multi-mycotoxin method based on liquid chromatography–tandem mass spectrometry (LC-MS/MS). Both F. sporotrichioides and F. langsethiae synthesised type-A trichothecenes, i.e. T-2 and HT-2 toxins, diacetoxyscirpenol (DAS) and neosolaniol (NEO). In addition, both species could be verified as beauvericin producers. The toxin production occurred in both cereal-based assays but was more predominant on the carbohydrate-rich rice-flour medium. The two species were potent producers of T-2 toxin, the highest amounts measured being at a level of 20,000?μg/kg after 7 days-incubation. Differences between the species were observed regarding the quantitative production of the other trichothecenes: F. sporotrichioides was a more prolific producer of HT-2 toxin and beauvericin, whereas F. langsethiae produced higher amounts of DAS and NEO. On rice-flour assay, the toxin production was monitored during the growth period. The production started rapidly at an early growth phase and several toxins could be detected already after the 1st day of incubation, the highest concentrations being at mg/kg level. The results also indicated that the biosynthesis by F. sporotrichioides and F. langsethiae shifted towards the other type-A trichothecenes at the expense of T-2 toxin at the end of the cultivation.