In vitro selection of cell-internalizing 2′-modified RNA aptamers against Pseudomonas aeruginosa

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• 作者：A. S. Davydova ; M. A. Vorobyeva ; M. R. Kabilov…
• 关键词：RNA aptamers ; 2′-fluoro RNA ; in vitro selection ; cell internalizing aptamers ; antibacterial aptamers
• 刊名：Russian Journal of Bioorganic Chemistry
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：43
• 期：1
• 页码：58-63
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biochemistry, general; Bioorganic Chemistry; Organic Chemistry; Biomedicine general;
• 出版者：Pleiades Publishing
• ISSN：1608-330X
• 卷排序：43

文摘

Infectious diseases caused by bacterial or viral agents represent the major cause of human pathogenesis and mortality worldwide. A development of novel antibacterial therapeutics and diagnostic tools is a very acute task. The use of DNA and RNA aptamers targeted to certain bacteria could be a promising solution to this problem. Here, we propose a new protocol of selection of 2′-fluoro RNA aptamers capable to internalize into bacterial cells. Using whole-cell SELEX against Pseudomonas aeruginosa, enriched 2′-fluoro RNA library was obtained, and its sequencing and data analysis were fulfilled. It was found that the central region of predominating aptamer sequence is identical to the fragment of P. aeruginosa rRNA. A possibility of internalizing of this aptamer into bacterial cells is shown. It is hypothesized that aptamers could be internalized more effectively as heterodimeric complexes.