In-depth LC-MS/MS analysis of the chicken ovarian cancer proteome reveals conserved and novel differentially regulated proteins in humans

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• 作者：Angelito I. Nepomuceno ; Huanjie Shao ; Kai Jing…
• 关键词：Mass spectrometry ; Proteomics ; Ovarian Cancer ; Chicken ; Ovostatin 2 ; OVOS2
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：407
• 期：22
• 页码：6851-6863
• 全文大小：1,589 KB
• 参考文献：1.Bast RC, Hennessy B, Mills GB (2009) The biology of ovarian cancer: new opportunities for translation. Nat Rev Cancer 9:415-28CrossRef
  2.American Cancer Society (2015) Cancer Facts & Figures 2015. American Cancer Society, Atlanta
  3.Williams TI, Toups KL, Saggese DA, Kalli KR, Cliby WA, Muddiman DC (2007) Epithelial ovarian cancer: disease etiology, treatment, detection, and investigational gene, metabolite, and protein biomarkers. J Proteome Res 6:2936-962CrossRef
  4.Cho KR, Shih Ie M (2009) Ovarian cancer. Annu Rev Pathol 4:287-13CrossRef
  5.Vanderhyden BC, Shaw TJ, Ethier JF (2003) Animal models of ovarian cancer. Reprod Biol Endocrinol 1:1-1CrossRef
  6.Garson K, Shaw TJ, Clark KV, Yao DS, Vanderhyden BC (2005) Models of ovarian cancer—are we there yet? Mol Cell Endocrinol 239:15-6CrossRef
  7.Johnson PA, Giles JR (2013) The hen as a model of ovarian cancer. Nat Rev Cancer 13:432-36CrossRef
  8.Hawkridge AM (2014) The chicken model of spontaneous ovarian cancer. Proteomics Clin Appl 8:689-99CrossRef
  9.Fredrickson TN (1987) Ovarian tumors of the hen. Environ Health Perspect 73:35-1CrossRef
  10.Fathalla MF (1971) Incessant ovulation—a factor in ovarian neoplasia? Lancet 2:163CrossRef
  11.Jackson E, Anderson K, Ashwell C, Petitte J, Mozdziak PE (2007) CA125 expression in spontaneous ovarian adenocarcinomas from laying hens. Gynecol Oncol 104:192-98CrossRef
  12.Hakim AA, Barry CP, Barnes HJ, Anderson KE, Petitte J, Whitaker R, Lancaster JM, Wenham RM, Carver DK, Turbov J, Berchuck A, Kopelovich L, Rodriguez GC (2009) Ovarian adenocarcinomas in the laying hen and women share similar alterations in p53, ras, and HER-2/neu. Cancer Prev Res 2:114-21CrossRef
  13.Ansenberger K, Zhuge Y, Lagman JA, Richards C, Barua A, Bahr JM, Hales DB (2009) E-cadherin expression in ovarian cancer in the laying hen, Gallus domesticus, compared to human ovarian cancer. Gynecol Oncol 113:362-69CrossRef
  14.Gonzalez Bosquet J, Peedicayil A, Maguire J, Chien J, Rodriguez GC, Whitaker R, Petitte JN, Anderson KE, Barnes HJ, Shridhar V, Cliby WA (2011) Comparison of gene expression patterns between avian and human ovarian cancers. Gynecol Oncol 120:256-64CrossRef
  15.Hawkridge AM, Muddiman DC (2009) Mass spectrometry–based biomarker discovery: toward a global proteome index of individuality. Annu Rev Anal Chem 2:265-77CrossRef
  16.Hawkridge AM, Wysocky RB, Petitte JN, Anderson KE, Mozdziak PE, Fletcher OJ, Horowitz JM, Muddiman DC (2010) Measuring the intra-individual variability of the plasma proteome in the chicken model of spontaneous ovarian adenocarcinoma. Anal Bioanal Chem 398:737-49CrossRef
  17.Andrews Kingon GL, Petitte JN, Muddiman DC, Hawkridge AM (2013) Multi-peptide nLC-PC-IDMS-SRM-based assay for the quantification of biomarkers in the chicken ovarian cancer model. Methods 61:323-30CrossRef
  18.Shevchenko A, Wilm M, Vorm O, Mann M (1996) Mass spectrometric sequencing of proteins from silver-staining polyacrylamide gels. Anal Chem 68:850-58CrossRef
  19.Andrews GL, Shuford CM, Burnett JC Jr, Hawkridge AM, Muddiman DC (2009) Coupling of a vented column with splitless nanoRPLC-ESI-MS for the improved separation and detection of brain natriuretic peptide-32 and its proteolytic peptides. J Chromatogr B Anal Technol Biomed Life Sci 877:948-54CrossRef
  20.Nesvizhskii AI, Keller A, Kolker REA (2003) A statistical model for identifying proteins by tandem mass spectrometry. Anal Chem 75:4646-658CrossRef
  21.Keller A, Nesvizhskii AI, Kolker E, Aebersold R (2002) Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search. Anal Chem 74:5383-392CrossRef
  22.Weatherly DB, Atwood JA 3rd, Minning TA, Cavola C, Tarleton RL, Orlando R (2005) A Heuristic method for assigning a false-discovery rate for protein identifications from Mascot database search results. Mol Cell Proteomics 4:762-72CrossRef
  23.Zybailov B, Mosley AL, Sardiu ME, Coleman MK, Florens L, Washburn MP (2006) Statistical analysis of membrane proteome expression changes in Saccharomyces cerevisiae research articles. J Proteome Res 5:2339-347CrossRef
  24.Teves ME, Zhang Z, Costanzo RM, Henderson SC, Corwin FD, Zweit J, Sundaresan G, Subler M, Salloum FN, Rubin BK, Strauss JF 3rd (2013) Sperm-associated antigen-17 gene is essential for motile cilia function and neonatal survival. Am J Respir Cell Mol Biol 48:765-72CrossRef
  25.Wu J, Mukherjee A, Lebman DA, Fang X (2012) Gene expression of the lysophosphatidic acid receptor 1 is a target of transforming growth factor beta. Oncogene 32(26):3198-206CrossRef
  26.Fang X, Yu S, Bast RC, Liu S, Xu HJ, Hu SX, LaPushin R, Claret FX, Aggarwal BB, Lu Y, Mills GB (2004) Mechanisms for lysophosphatidic acid-induced cytokine production in ovarian cancer cells. J Biol Chem 279:9653-661CrossRef
  27.Liu HB, Sadygov RG, Yates JR (2004) A model for random sampling and estimation of relative pro
• 作者单位：Angelito I. Nepomuceno (1)
  Huanjie Shao (3)
  Kai Jing (3)
  Yibao Ma (3)
  James N. Petitte (2)
  Michael O. Idowu (4)
  David C. Muddiman (1)
  Xianjun Fang (3)
  Adam M. Hawkridge (5) (6)
  
  1. W.M. Keck FTMS Laboratory for Human Health Research, Department of Chemistry, North Carolina State University, 2620 Yarbrough Dr., Box 8204, Raleigh, NC, 27695, USA
  3. Department of Biochemistry and Molecular Biology, Virginia Commonwealth University, 1101 E. Marshall Street, Richmond, VA, 23298, USA
  2. Prestage Department of Poultry Science, North Carolina State University, 2711 Founders Drive, Raleigh, NC, 27695, USA
  4. Department of Pathology, Virginia Commonwealth University, 1101 E. Marshall Street, Richmond, VA, 23298, USA
  5. Department of Pharmaceutics, Virginia Commonwealth University, 410 North 12th Street Smith Building, Richmond, VA, 23298, USA
  6. Department of Pharmacotherapy & Outcomes Science, Virginia Commonwealth University, 410 North 12th Street Smith Building, Richmond, VA, 23298, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

Ovarian cancer (OVC) remains the most lethal gynecological malignancy in the world due to the combined lack of early-stage diagnostics and effective therapeutic strategies. The development and application of advanced proteomics technology and new experimental models has created unique opportunities for translational studies. In this study, we investigated the ovarian cancer proteome of the chicken, an emerging experimental model of OVC that develops ovarian tumors spontaneously. Matched plasma, ovary, and oviduct tissue biospecimens derived from healthy, early-stage OVC, and late-stage OVC birds were quantitatively characterized by label-free proteomics. Over 2600 proteins were identified in this study, 348 of which were differentially expressed by more than twofold (p?≤-.05) in early- and late-stage ovarian tumor tissue specimens relative to healthy ovarian tissues. Several of the 348 proteins are known to be differentially regulated in human cancers including B2M, CLDN3, EPCAM, PIGR, S100A6, S100A9, S100A11, and TPD52. Of particular interest was ovostatin 2 (OVOS2), a novel 165-kDa protease inhibitor found to be strongly upregulated in chicken ovarian tumors (p--.0005) and matched plasma (p--.003). Indeed, RT-quantitative PCR and Western blot analysis demonstrated that OVOS2 mRNA and protein were also upregulated in multiple human OVC cell lines compared to normal ovarian epithelia (NOE) cells and immunohistochemical staining confirmed overexpression of OVOS2 in primary human ovarian cancers relative to non-cancerous tissues. Collectively, these data provide the first evidence for involvement of OVOS2 in the pathogenesis of both chicken and human ovarian cancer.