Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores
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- 作者：Yan-Mei Qiao ; Yong-Chao Guo ; Xian-En Zhang ; Ya-Feng Zhou ; Zhi-Ping Zhang ; Hong-Ping Wei ; Rui-Fu Yang and Dian-Bing Wang
- 关键词：Bacillus anthracis ; Detection ; Isothermal amplification ; LAMP
- 刊名：Biotechnology Letters
- 出版年：2007
- 出版时间：December, 2007
- 年：2007
- 卷：29
- 期：12
- 页码：1939-1946
- 全文大小：228 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Microbiology
Biotechnology
Applied Microbiology
Biochemistry - 出版者：Springer Netherlands
- ISSN：1573-6776
文摘
A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthracis species. The detection limits of the LAMP assay were 10 spores/tube for pure cultures and 100 spores/2 mg powder for simulated powder samples. The results show that the LAMP protocol is a promising method for detecting B. anthracis.