Biosynthesis of wine aroma: transcript profiles of hydroxymethylbutenyl diphosphate reductase, geranyl diphosphate synthase, and linalool/nerolidol synthase parallel monoterpenol glycoside accumulation in Gewürztraminer grapes

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• 作者：Diane M. Martin (1) (2)
  Angela Chiang (1) (2)
  Steven T. Lund (2)
  J?rg Bohlmann (1) (2)
  
• 关键词：Vitis vinifera ; Wine grape ; Monoterpenoids ; Terpene synthases ; Precursor volatile organic compounds
• 刊名：Planta
• 出版年：2012
• 出版时间：September 2012
• 年：2012
• 卷：236
• 期：3
• 页码：919-929
• 全文大小：469KB
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• 作者单位：Diane M. Martin (1) (2)
  Angela Chiang (1) (2)
  Steven T. Lund (2)
  J?rg Bohlmann (1) (2)
  
  1. Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, BC, V6T 1Z4, Canada
  2. Wine Research Centre, University of British Columbia, 2205 East Mall, Vancouver, BC, V6T 1Z4, Canada
  

文摘

In developing grapevine (Vitis vinifera L.) berries, precursor volatile organic compounds (PVOCs) are largely stored as glycosides which may be hydrolyzed to release VOCs during fruit ripening, wine making, or aging. VOCs can be further transformed by yeast metabolism. Together, these processes contribute to complexity of wine aromas. Floral and citrus odors of many white wine varietals are attributed to monoterpenes and monoterpene alcohols, while phenolic compounds, norisoprenoids, and other volatiles also play important roles in determining aroma. We present an analysis of PVOCs stored as glycosides in developing Gewürztraminer berries during the growing season. We optimized a method for PVOC analysis suitable for small amounts of Muscat grapevine berries and showed that the amount of PVOCs dramatically increased during and after véraison. Transcript profiling of the same berry samples underscored the involvement of terpenoid pathway genes in the accumulation of PVOCs. The onset of monoterpenol PVOC accumulation in developing grapes was correlated with an increase of transcript abundances of early terpenoid pathway enzymes. Transcripts encoding the methylerythritol phosphate pathway gene 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, as well as geraniol diphosphate synthase, were up-regulated preceding and during the increase in monoterpenol PVOCs. Transcripts for linalool/nerolidol synthase increased in later véraison stages.