Characterization of mitochondrial dicarboxylate/tricarboxylate transporters from grape berries
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- 作者：Ana Regalado (1)
Ciro Leonardo Pierri (2)
Maria Bitetto (2)
Valentina Liliana Laera (2)
Catarina Pimentel (1)
Rita Francisco (1)
José Passarinho (3)
Maria M. Chaves (1) (3)
Gennaro Agrimi (2) - 关键词：Grape berry ripening ; Malate ; Mitochondrial carriers ; Mitochondrial di/tricarboxylate transporters ; TCA cycle ; Vitis
- 刊名：Planta
- 出版年：2013
- 出版时间：March 2013
- 年：2013
- 卷：237
- 期：3
- 页码：693-703
- 全文大小：791KB
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Ciro Leonardo Pierri (2)
Maria Bitetto (2)
Valentina Liliana Laera (2)
Catarina Pimentel (1)
Rita Francisco (1)
José Passarinho (3)
Maria M. Chaves (1) (3)
Gennaro Agrimi (2)
1. Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República-EAN, 2780-157, Oeiras, Portugal
2. Department of Biosciences, Biotechnology and Pharmacological Sciences, University of Bari, Via E. Orabona 4, 70125, Bari, Italy
3. Instituto Nacional de Recursos Biológicos, I.P., Quinta do Marquês, 2784-505, Oeiras, Portugal
文摘
Grape berries (Vitis vinifera L fruit) exhibit a double-sigmoid pattern of development that results from two successive periods of vacuolar swelling during which the nature of accumulated solutes changes significantly. Throughout the first period, called green or herbaceous stage, berries accumulate high levels of organic acids, mainly malate and tartrate. At the cellular level fruit acidity comprises both metabolism and vacuolar storage. Malic acid compartmentation is critical for optimal functioning of cytosolic enzymes. Therefore, the identification and characterization of the carriers involved in malate transport across sub-cellular compartments is of great importance. The decrease in acid content during grape berry ripening has been mainly associated to mitochondrial malate oxidation. However, no Vitis vinifera mitochondrial carrier involved in malate transport has been reported to date. Here we describe the identification of three V. vinifera mitochondrial dicarboxylate/tricarboxylate carriers (VvDTC1-3) putatively involved in mitochondrial malate, citrate and other di/tricarboxylates transport. The three VvDTCs are very similar, sharing a percentage of identical residues of at least 83?%. Expression analysis of the encoding VvDTC genes in grape berries shows that they are differentially regulated exhibiting a developmental pattern of expression. The simultaneous high expression of both VvDTC2 and VvDTC3 in grape berry mesocarp close to the onset of ripening suggests that these carriers might be involved in the transport of malate into mitochondria.