Phylogenetic study of Japanese Dickeya spp. and development of new rapid identification methods using PCR–RFLP
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- 作者：Radix Suharjo (1) (2)
Hiroyuki Sawada (3)
Yuichi Takikawa (1) - 关键词：Biochemical assays ; Dickeya strains ; Genomic fingerprinting ; MLSA (multilocus sequence analysis) ; PCR–RFLP
- 刊名：Journal of General Plant Pathology
- 出版年：2014
- 出版时间：May 2014
- 年：2014
- 卷：80
- 期：3
- 页码：237-254
- 全文大小：
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Hiroyuki Sawada (3)
Yuichi Takikawa (1)
1. Laboratory of Plant Pathology, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Shizuoka, 422-8529, Japan
2. Faculty of Agriculture, University of Lampung, Jl. Prof. Sumantri Brodjonegoro I, Gedong Meneng, Bandar Lampung, 35145, Indonesia
3. National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan - ISSN：1610-739X
文摘
Forty-one representative Japanese Dickeya spp. (Erwinia chrysanthemi) strains isolated from 24 plants in Japan were investigated using multilocus sequence analysis of recA, dnaX, rpoD, gyrB and 16S rDNA; PCR–RFLP (restriction fragment length polymorphism) of recA, rpoD and gyrB genes; PCR genomic fingerprinting; and biochemical tests. Based on the recA, dnaX, rpoD, gyrB and 16S rDNA sequences and PCR genomic fingerprinting, the strains were essentially divided into six groups (I–VI). Group I corresponded to D. chrysanthemi, group II corresponded to D. dadantii, group III to D. dianthicola and group IV to D. zeae. Meanwhile, group V and group VI could not be assigned to any existing Dickeya species, and they were deduced to be two putative new species. The PCR–RFLP analysis of gyrB, rpoD and recA clearly differentiated the six groups of Dickeya strains. From the results of the biochemical tests, the strains were assigned to biovars 1, 3, 5, 8 and 9; only one strain (SUPP 2525) was not assignable to the existing biovars. We also showed that the PCR–RFLP analysis of rpoD, gyrB and recA can be used as a rapid technique to identify Japanese Dickeya strains.