Infection by a coronatine-producing strain of Pectobacterium cacticidum isolated from sunflower plants in Mexico is characterized by soft rot and chlorosis

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• 作者：José Humberto Valenzuela-Soto…
• 关键词：Pectobacterium cacticidum ; Sunflower ; Soft rot ; Chlorosis ; Plant ; cell ; wall ; degrading enzymes ; Coronatine
• 刊名：Journal of General Plant Pathology
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：81
• 期：5
• 页码：368-381
• 全文大小：3,069 KB
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• 作者单位：José Humberto Valenzuela-Soto (5)
  Luis David Maldonado-Bonilla (2)
  Gustavo Hernández-Guzmán (3)
  Gabriel Rincón-Enríquez (4)
  Norma Angélica Martínez-Gallardo (1)
  Enrique Ramírez-Chávez (1)
  Ismael Cisneros Hernández (1)
  José Luis Hernández-Flores (1)
  John Paul Délano-Frier (1)
  
  5. Departamento de Plásticos en la Agricultura, Centro de Investigación en Química Aplicada, Boulevard Enrique Reyna Hermosillo 140, 25294, Saltillo, Coahuila, Mexico
  2. División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, Col. Lomas 4 sección, 78216, San Luis Potosí, San Luis Potosí, Mexico
  3. División de Ciencias de la Vida, Departamento de Alimentos, Universidad de Guanajuato, Campus Irapuato-Salamanca, Km 9, Carretera Irapuato-Silao, Col. El Copal, 36500, Irapuato, Guanajuato, Mexico
  4. Unidad de Biotecnología Vegetal. Centro de Investigación y Asistencia en Tecnología y Dise?o del Estado de Jalisco, A.C. (CIATEJ), Avenida Normalistas número 800, Colonia Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico
  1. Unidad de Biotecnología e Ingeniería Genética de Plantas, (Cinvestav, Unidad Irapuato), Km 9.6 del Libramiento Norte Carretera Irapuato-León, Apartado Postal 629, 36821, Irapuato, Guanajuato, Mexico
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Pathology
  Agriculture
  Microbiology
  
• 出版者：Springer Japan
• ISSN：1610-739X

文摘

A novel strain of Pectobacterium, isolated from infected sunflower plants (Helianthus annuus L.) in Mexico was characterized. Inoculated sunflower plants developed both tissue chlorosis and soft-rot on leaves. The broad host range shown by this pathogen, which included members of the Agavaceae, Asteraceae, Brassicaceae and Solanaceae, was characteristic of the genus Pectobacterium. The metabolic profile and molecular data, in addition to the secretion of plant-cell-wall-degrading enzymes, confirmed its identification as a member of the Pectobacterium genus. A phylogenetic tree constructed on the basis of its 16S rDNA sequence revealed a high identity (98 %) with P. cacticidum. Amplification and restriction-fragment-length-polymorphism analysis of internal transcribed sequences further confirmed its classification as P. cacticidum. Similar to strains of P. atrosepticum, P. carotovorum and Dickeya spp., the new P. cacticidum strain FHLGJ22 has a coronafacic ligase gene and produces coronatine, a virulence factor usually associated with phytopathogenic strains of Pseudomonas syringae. Our results suggest that this strain may utilize a dual infection process involving cell maceration and plant toxins, which is synchronized via an unknown mechanism, might confer an adaptive advantage to colonize different plant hosts. Keywords Pectobacterium cacticidum Sunflower Soft rot Chlorosis Plant-cell-wall-degrading enzymes Coronatine