Cardinal temperature differences, determined in vitro, between closely related species and subspecies of pectinolytic bacteria responsible for blackleg and soft rot on potatoes

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• 作者：S. du Raan ; T. A. Coutinho ; J. E. van der Waals
• 关键词：Pectobacterium ; Dickeya ; Growth ; Optimal temperature ; Temperature range ; Pectinolytic bacteria
• 刊名：European Journal of Plant Pathology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：144
• 期：2
• 页码：361-369
• 全文大小：511 KB
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• 作者单位：S. du Raan (1)
  T. A. Coutinho (2)
  J. E. van der Waals (3)
  
  1. Department of Microbiology and Plant Pathology, University of Pretoria, Private Bag X20, Hatfield, 0028, South Africa
  2. Department of Microbiology and Plant Pathology, Forestry and Agricultural Institute (FABI), University of Pretoria, Private Bag X20, Hatfield, 0028, South Africa
  3. Department of Microbiology and Plant Pathology, University of Pretoria, Private Bag X20, Hatfield, 0028, South Africa
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Pathology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-8469

文摘

Potato blackleg and soft rot cause major losses and are caused by two bacterial genera, Pectobacterium and Dickeya. Species affecting potatoes are Pectobacterium atrosepticum (Pba), Pectobacterium carotovorum subsp. carotovorum (Pcc), Pectobacterium carotovorum subsp. brasiliense (Pcb), Pectobacterium wasabiae (Pwa), Dickeya dadantii (Dda) and Dickeya solani (Dso). Pathogenicity of these species is dependent on temperature, with each species having its own optimal temperature and temperature range for growth, leading to varying degrees of losses. Pectobacterium atrosepticum, a temperature sensitive species, mainly occurs in temperate climates, Pcc in temperate to tropical, and Dickeya spp. in subtropical environments. The aim of this study was to determine the cardinal growth temperatures for the species responsible for blackleg and soft rot in vitro. Bacterial isolates were incubated in a temperature gradient shaking incubator at 30 different temperatures ranging from 卤5 掳C to 卤56 掳C, and growth measured at two set time intervals. Results were statistically analysed using the Gaussian function. The optimal temperature of 31 掳C and temperature range of 20 掳C to 38 掳C for Pectobacterium carotovorum subsp. brasiliense, was similar to those recorded for Pcc. Pectobacterium wasabiae grew at an optimal temperature of 29 掳C and range of 20 掳C to 34 掳C. Higher optimal temperatures of 32 掳C and 34 掳C, with ranges of 21 掳C to 38 掳C and 23 掳C to 41 掳C were recorded for Dda and Dso, respectively. The minimal variation in optimal temperatures between different species might be an indication that temperature ranges, rather than optimal temperature, play an important role in disease development. Results for Dso, which has not yet been reported in South Africa, are especially important in light of prevailing temperatures in South African potato production regions. Keywords Pectobacterium Dickeya Growth Optimal temperature Temperature range, Pectinolytic bacteria