Mangrove Helps: Sonneratia alba-Synthesized Silver Nanoparticles Magnify Guppy Fish Predation Against Aedes aegypti Young Instars and Down-Regulate the Expression of Envelope (E) Gene in Dengue Virus (Serotype DEN-2)

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• 作者：Kadarkarai Murugan ; Devakumar Dinesh ; Manickam Paulpandi…
• 关键词：Arbovirus ; Biosafety ; Green ; synthesis ; Poecilia reticulata ; Sonneratia alba
• 刊名：Journal of Cluster Science
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：28
• 期：1
• 页码：437-461
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Catalysis; Inorganic Chemistry; Physical Chemistry; Nanochemistry;
• 出版者：Springer US
• ISSN：1572-8862
• 卷排序：28

文摘

The control of dengue vectors with effective tools is crucial. Here, we fabricated silver nanoparticles (AgNP) using a cheap method relying to a mangrove extract (Sonneratia alba) as a reducing and stabilizing agent. AgNP were characterized by UV–vis spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray diffraction. LC₅₀ of S. alba extract against Aedes aegypti ranged from 192.03 ppm (larva I) to 353.36 ppm (pupa). LC₅₀ of AgNP ranged from 3.15 (I) to 13.61 ppm (pupa). Sub-lethal doses of AgNP magnified predation rates of guppy fishes, Poecilia reticulata, against Ae. aegypti and Chironomus kiiensis larvae. Mangrove-fabricated AgNP were evaluated for their antimicrobial potential against Bacillus subtilis, Klebsiella pneumoniae, and Salmonella typhi, using the agar disc diffusion and minimum inhibitory concentration protocol. Notably, S. alba-synthesized AgNP tested at doses ranging from 5 to 15 µg/mL down-regulated the expression of the envelope (E) gene and protein in dengue virus (serotype DEN-2), while only little cytotoxicity rates (i.e. <15%) were detected on Vero cells when AgNP were tested at 10 µg/mL. Overall, this study pointed out the potential of S. alba-synthesized AgNP to develop eco-friendly nanoformulations effective against dengue virus and its mosquito vectors.