The seasonal dynamics of diarrhetic shellfish poisoning toxins in the digestive gland of Crenomytilus grayanus (Dunker, 1853) analyzed by high-performance liquid chromatography with fluorometric detection
详细信息 查看全文
- 作者：P. A. Kameneva ; T. Yu. Orlova ; A. B. Imbs
- 刊名：Russian Journal of Marine Biology
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：42
- 期：3
- 页码：258-265
- 全文大小：445 KB
- 刊物主题：Freshwater & Marine Ecology;
- 出版者：Springer US
- ISSN：1608-3377
- 卷排序：42
文摘
The seasonal dynamics of diarrhetic shellfish poisoning (DSP) toxins in the digestive gland of the mussel Crenomytilus grayanus (Dunker, 1853) from Peter the Great Bay in the Sea of Japan have been studied. Toxins in the form of 4-bromomethyl-7-methoxycoumarin derivatives have been analyzed using high-performance liquid chromatography (HPLC) with fluorometric detection. This method allows the determination of the quantitative and qualitative composition of individual toxins. All the C. grayanus samples contained DSP toxins, but their concentration varied substantially during the year. The highest concentration was recorded from the mussels collected in May; the lowest concentration was recorded in February. The toxins were represented predominantly by dinophysistoxin-2; in July-September and December, okadaic acid was detected. An assumption has been made about the relationship between the potential producers of DSP toxins in the waters of the Peter the Great Bay and the dynamics of these toxins in tissues of C. grayanus. The HPLC method, as an alternative to the enzyme-linked immunosorbent assay, was used to assess the quantitative and qualitative composition of DSP toxins in seafood for the first time in the Russian Federation. This method can be recommended for the routine monitoring of toxins conducted by the controlling organizations of the Russian Federal Service for Veterinary and Phytosanitary Surveillance.Keywordsdiarrhetic shellfish poisoningokadaic aciddinophysistoxin-2Crenomytilus grayanusHPLCfluorometric detection