Effect of changes in the deuterium content of drinking water on the hydrogen isotope ratio of urinary steroids in the context of sports drug testing

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• 作者：Thomas Piper (1) (3)
  Karoline Degenhardt (1)
  Eugen Federherr (2)
  Andreas Thomas (3)
  Mario Thevis (3)
  Martial Saugy (1)
  
• 关键词：Hydrogen isotope ratio ; GC–TC–IRMS ; Excretion study ; Steroids ; Enriched drinking water ; Exchangeable hydrogen
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2013
• 出版时间：March 2013
• 年：2013
• 卷：405
• 期：9
• 页码：2911-2921
• 全文大小：362KB
• 参考文献：1. Piper T, Thomas A, Thevis M, Saugy M (2012) Drug Test Anal 4:717-27 CrossRef
  2. Coplen TB (2011) Rapid Commun Mass Spectrom 25:2538-560
  3. Piper T, Baume N, Strahm E, Emery C, Saugy M (2012) Steroids 77:644-54 CrossRef
  4. Solberg HE (1993) J Int Fed Clin Chem 5:162-65
  5. Flenker U, Güntner U, Sch?nzer W (2008) Steroids 73:408-16 CrossRef
  6. Piper T, Mareck U, Geyer H, Flenker U, Thevis M, Platen P, Sch?nzer W (2008) Rapid Commun Mass Spectrom 22:2161-175 CrossRef
  7. Piper T, Opfermann G, Thevis M, Sch?nzer W (2010) Rapid Commun Mass Spectrom 24:3171-181 CrossRef
  8. Friedman I (1953) Geochim Cosmochim Acta 4:89-03 CrossRef
  9. Bowen GJ, Winter DA, Spero HJ, Zierenberg RA, Reeder MD, Cerling TE, Ehleringer JR (2005) Rapid Commun Mass Spectrom 19:3442-450 CrossRef
  10. Brencic M, Vreca P (2006) Rapid Commun Mass Spectrom 20:3205-212 CrossRef
  11. Bowen GJ, Ehleringer JR, Chesson LA, Stange E, Cerling TE (2007) Water Resour Res 43:W03419 CrossRef
  12. Kim GE, Ryu JS, Shin WJ, Bong YS, Lee KS, Choi MS (2012) Rapid Commun Mass Spectrom 26:195-04 CrossRef
  13. Data available at: http://wateriso.eas.purdue.edu/waterisotopes/index.html (accessed 27.07.2012)
  14. Koehler K, Braun H, De Marees M, Fusch G, Fusch C, Mester J, Sch?nzer W (2010) J Sports Sci 13:1435-449 CrossRef
  15. Mareck U, Geyer H, Opfermann G, Thevis M, Sch?nzer W (2008) J Mass Spectrom 43:877-91 CrossRef
  16. Piper T, Thevis M, Flenker U, Sch?nzer W (2009) Rapid Commun Mass Spectrom 23:1917-926 CrossRef
  17. Werner RA, Brand WA (2001) Rapid Commun Mass Spectrom 15:501-19 CrossRef
  18. Docherty G, Jones V, Evershed P (2001) Rapid Commun Mass Spectrom 15:730-38 CrossRef
  19. Morrison J, Brockwell T, Merren T, Fourel F, Phillips AM (2001) Anal Chem 73:3570-575 CrossRef
  20. Meier-Augenstein W (2010) In: Meier-Augenstein W (ed) Stable isotope forensics: an introduction to the forensic application of stable isotope analysis. Wiley, Oxford, pp 85-0
  21. Schoeller DA, Colligan AS, Shriver T, Avak H, Bartok-Olson C (2000) J Mass Spectrom 35:1128-132 CrossRef
  22. London IM, Rittenberg D (1950) J Biol Chem 184:687-91
  23. Goodman DS, Noble RP (1968) J Clin Invest 47:231-41 CrossRef
  24. Goodman DWS, Smith FR, Seplowitz AH, Ramakrishnan R, Dell RB (1980) J Lipid Res 21:699-13
  25. Neese RA, Faix D, Kletke C, Wu K, Wang AC, Shackleton CHL, Hellerstein MK (1993) Am J Physiol Endocrinol Metab 264:E136–E147
  26. Zimmerman J (1986) Untersuchungen zum Nachweis von exogenen Gaben von Testosteron. Thesis, Deutsche Sporthochschule K?ln, Cologne
  27. Rauth S (1994) Referenzbereiche von urin?ren Steroidkonzentrationen und Steroidquotienten. Thesis, Deutsche Sporthochschule K?ln, Cologne
  28. Schoenheimer R, Rittenberg D (1937) J Biol Chem 121:235-53
  29. Halford JO, Anderson LC, Bates JR (1934) J Am Chem Soc 56:491-92 CrossRef
  30. Vestergaard P (1978) Acta Endocrinol Suppl 217:96-20
  
• 作者单位：Thomas Piper (1) (3)
  Karoline Degenhardt (1)
  Eugen Federherr (2)
  Andreas Thomas (3)
  Mario Thevis (3)
  Martial Saugy (1)
  
  1. Swiss Laboratory for Doping Analysis, University Center of Legal Medicine, Geneva and Lausanne, Centre Hospitalier Universitaire Vaudois and University Lausanne, Ch. des Croisettes 22, 1066, Epalinges, Switzerland
  3. German Sport University Cologne, Center for Preventive Doping Research—Institute of Biochemistry, Am Sportpark Müngersdorf 6, 50933, K?ln, Germany
  2. Elementar Analysensysteme GmbH, Donaustr. 7, 63452, Hanau, Germany
  
• ISSN：1618-2650

文摘

The hydrogen isotope ratio (HIR) of body water and, therefore, of all endogenously synthesized compounds in humans, is mainly affected by the HIR of ingested drinking water. As a consequence, the entire organism and all of its synthesized substrates will reflect alterations in the isotope ratio of drinking water, which depends on the duration of exposure. To investigate the effect of this change on endogenous urinary steroids relevant to doping-control analysis the hydrogen isotope composition of potable water was suddenly enriched from -50 to 200 -and maintained at this level for two weeks for two individuals. The steroids under investigation were 5β-pregnane-3α,20α-diol, 5α-androst-16-en-3α-ol, 3α-hydroxy-5α-androstan-17-one (ANDRO), 3α-hydroxy-5β-androstan-17-one (ETIO), 5α-androstane-3α,17β-diol, and 5β-androstane-3α,17β-diol (excreted as glucuronides) and ETIO, ANDRO and 3β-hydroxyandrost-5-en-17-one (excreted as sulfates). The HIR of body water was estimated by determination of the HIR of total native urine, to trace the induced changes. The hydrogen in steroids is partly derived from the total amount of body water and cholesterol-enrichment could be calculated by use of these data. Although the sum of changes in the isotopic composition of body water was 150 - shifts of approximately 30 -were observed for urinary steroids. Parallel enrichment in their HIR was observed for most of the steroids, and none of the differences between the HIR of individual steroids was elevated beyond recently established thresholds. This finding is important to sports drug testing because it supports the intended use of this novel and complementary methodology even in cases where athletes have drunk water of different HIR, a plausible and, presumably, inevitable scenario while traveling.