SSAP analysis reveals candidate genes associated with deastringency in persimmon (Diospyros kaki Thunb.) treated with 40?°C water

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• 作者：Changfei Guan ; Li Chen ; Wenxing Chen ; Rongli Mo ; Qinglin Zhang…
• 关键词：Persimmon ; 40?°C water ; Deastringency ; SSAP ; Transcript ; derived fragments ; qRT ; PCR
• 刊名：Tree Genetics & Genomes
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：11
• 期：2
• 全文大小：2,042 KB
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• 刊物主题：Forestry; Plant Genetics & Genomics; Plant Breeding/Biotechnology; Tree Biology; Biotechnology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1614-2950

文摘

Warm water treatment is a simple and effective deastringency method for the persimmon fruit. The astringency in the ‘Eshi 1-persimmon, a Chinese pollination-constant and non-astringent (C-PCNA) cultivar, can be quickly removed in 40?°C water coupled with the activation of the retrotransposon DkRE1. In this study, transcriptional level changes in ‘Eshi 1-persimmon treated with 40?°C water were studied with a sequence-specific amplification polymorphism (SSAP) approach to investigate the genes associated with deastringency. The complementary DNA (cDNA)-SSAP analysis revealed that 283 differentially expressed transcript-derived fragments (TDFs) potentially involved in ‘Eshi 1-persimmon deastringency were successfully cloned and sequenced. In total, 116 differentially expressed TDFs were annotated as genes with known function, including four aldehyde metabolism genes and two pectin-related genes. Our data suggested that the tannin-pectin complex formation and the aldehyde-mediated coagulation effect contributed to the deastringency of the persimmon fruit treated with 40?°C water. Notably, three gene fragments, triosephosphate isomerase (TDF 175-3), pyruvate kinase (TDF 284-2), and neomenthol dehydrogenase (TDF 238-2), which are potentially involved in the 40?°C water coagulation effect were identified. To our knowledge, this report is the first to identify these gene fragments in the persimmon. The SSAP method was innovatively applied to gene discovery based on high-density polymorphisms. Expression analysis and transcriptome data confirmed the validity and accuracy of the cDNA-SSAP method for discovering new candidate genes. These successfully isolated genes could facilitate understanding of astringency removal in persimmon.