Characterization and Comparison of Protein and Peptide Profiles and their Biological Activities of Improved Common Bean Cultivars (Phaseolus vulgaris L.) from Mexico and Brazil

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• 作者：Luis Mojica ; Elvira Gonz谩lez de Mej铆a
• 关键词：伪 ; Amylase inhibitor ; Bioactive peptides ; Bowman ; Birk inhibitor ; Common beans ; Kunitz trypsin inhibitor ; Lectin ; Phaseolin ; Proteins
• 刊名：Plant Foods for Human Nutrition
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：70
• 期：2
• 页码：105-112
• 全文大小：766 KB
• 参考文献：1.Sathe SK, Deshpande SS (2003) Beans. In: Encyclopedia of Food Science Technology, Academic Press, London, UK, pp 403鈥?12
  2.Campos-Vega R, Reynoso-Camacho R, Pedraza-Aboytes G, Acosta-Gallegos JA, Guzman-Maldonado SH, Paredes-L贸pez O, Oomah BD, Loarca-Pina G (2009) Chemical composition and in vitro polysaccharide fermentation of different beans. J Food Sci 74(7):59鈥?5View Article
  3.Paredes-L贸pez O, Guevara-Lara F, Bello-P茅rez LA (2006) Los Alimentos M谩gicos de las Culturas Ind铆genas Mesoamericanas. Fondo de Cultura Econ贸mica 3:59鈥?1
  4.Celleno L, Tolaini MV, D鈥橝more A, Perricone NV, Preuss HG (2007) A dietary supplement containing standardized Phaseolus vulgaris extract influences body composition of overweight men and women. Int J Med Sci 4:45鈥?2View Article
  5.Oseguera-Toledo M, Gonzalez de Mejia E, Dia VP, Amaya-Llano S (2011) Common bean hydrolysates inhibit inflammation in LPS-induced macrophages through suppression of NF- 螝B pathways. Food Chem 127:1175鈥?185View Article
  6.Hern谩ndez-Saavedra D, Mendoza-S谩nchez M, Hern谩ndez-Montiel HL, Guzm谩n-Maldonado HS, Loarca-Pi帽a G, Salgado LM, Reynoso-Camacho R (2013) Cooked common beans (Phaseolus vulgaris) protect against 尾-cell damage in streptozotocin-induced diabetic rats. Plant Foods Hum Nutr 68:207鈥?12View Article
  7.Feregrino-Perez AA, Pi帽ol-Felis C, Gomez-Arbones X, Guevara-Gonz谩lez RG, Campos-Vega R, Acosta-Gallegos J, Loarca-Pi帽a G (2014) A non-digestible fraction of the common bean (Phaseolus vulgaris L.) induces cell cycle arrest and apoptosis during early carcinog茅nesis. Plant Foods Hum Nutr 69:248鈥?54View Article
  8.Pereira MP, Tavano (2014) Use of different spices as potential natural antioxidant additives on cooked beans (Phaseolus vulgaris). Increase of DPPH radical scavenging activity and total phenolic content. Plant Foods Hum Nutr 69:337鈥?43View Article
  9.Mojica L, Chen K, Gonzalez de Mejia E (2014) Impact of commercial precooking of common bean (Phaseolus vulgaris) on the generation of peptides, after pepsin鈥損ancreatin hydrolysis, capable to inhibit dipeptidyl peptidase-IV. J Food Sci 80:H188鈥揌198View Article
  10.L贸pez-Barrios JA, Guti茅rrez-Uribe S, Serna-Sald铆var S (2014) Bioactive peptides and hydrolysates from pulses and their potential use as functional ingredients. J Food Sci 79:273鈥?83View Article
  11.Luna-Vital DA, Mojica L, Gonzalez de Mejia E, Mendoza S, Loarca-Pina G (2015) Biological potential of protein hydrolysates and peptides from common bean (Phaseolus vulgaris L.): a review. Food Res Int. doi:10.鈥?016/鈥媕.鈥媐oodres.鈥?014.鈥?1.鈥?24
  12.Sarmadi A, Ismail A (2010) Antioxidative peptides from food proteins: a review. Peptides 31:1949鈥?956View Article
  13.Alves M, Chavez I, Carrilho D, Veloso M, Pinto CR (2010) Detection of novel trypsin inhibitors in the cotyledons of Phaseolus vulgaris seeds. J Plant Physiol 167:848鈥?54View Article
  14.Chan SY, Zhang Y, Ng TB (2013) Brown kidney bean Bowman-Birk trypsin inhibitor is heat and pH stable and exhibits anti-proliferative activity. Appl Biochem Biotechnol 169:1306鈥?314View Article
  15.Mittal A, Kansal R, Kalia V, Tripathi M, Gupta VK (2014) A kidney bean trypsin inhibitor with an insecticidal potential against Helicoverpa armigera and Spodoptera litura. Acta Physiol Plant 36:525鈥?39View Article
  16.Zaugg I, Magni C, Panzeri D, Maminati MG, Bollini R, Benrey B, Bacher S, Sparvoli F (2013) QUES, a new Phaseolus vulgaris genotype resistant to common bean weevils, contains the arcelin-8 allele coding for new lectin-related variants. Theor Appl Genet 126:647鈥?61View Article
  17.Marsolais F, Pajak A, Yin F et al (2010) Proteomic analysis of common bean seed with storage protein deficiency reveals up-regulation of sulfur-rich proteins and starch and raffinose metabolic enzymes, and down-regulation of the secretory pathway. J Proteomics 73:1587鈥?600View Article
  18.Silva-Sanchez C, de la Rosa AP B, Leon-Galvan MF, De Lumen BO, De Leon-Rodr谋guez A, Gonzalez de Mej谋a E (2008) Bioactive peptides in amaranth (Amaranthus hypochondriacus) seed. J Agric Food Chem 56:1233鈥?240View Article
  19.Dia VP, Gomez T, Vernaza G, Berhow M, Chang YK, Gonzalez de Mejia E (2012) Bowman-Birk and Kunitz protease inhibitors among antinutrients and bioactives modified by germination and hydrolysis in Brazilian soybean cultivar BRS 133. J Agric Food Chem 60:7886鈥?894View Article
  20.Meg铆as C, Yust M, Pedroche J, Lquari H, Giron-Calle J, Alaiz M (2009) Purification of an ACE inhibitory peptide after hydrolysis of sunflower (Helianthus annuus L.) protein isolates. J Agric Food Chem 52:1928鈥?932View Article
  21.de Souza-Rocha T, Real LMH, Chang YK, Gonz谩lez de Mej铆a E (2014) Impact of germination and enzymatic hydrolysis of cowpea bean (Vigna unguiculata) on the generation of peptides capable of inhibiting dipeptidyl peptidase IV. Food Res Int 64:799鈥?09View Article
  22.Rui X, Boye J, Ribereau S, Simpson B, Prasher S (2011) Comparative study of the composition and thermal properties of protein isolates prepared from nine Phaseolus vulgaris legume varieties. Food Res Int 44:2497鈥?504View Article
  23.Montoya C, Lalles J, Beebe S, Leterme P (2010) Phaseolin diversity as a possible strategy to improve the nutritional value of common beans. Food Res Int 43:443鈥?49View Article
  24.Carrasco-Castilla J, Hernandez-Alvarez A, Jimenez-Martinez C, Jacinto-Hernandez C, Alaiz M, Giron-Calle J, Vioque J, Davila-Ortiz G (2012) Antioxidant and metal chelating activities of Phaseolus vulgaris L. var. Jamapa protein isolates, phaseolin and lectin hydrolysates. Food Chem 131:1157鈥?164View Article
  25.Tang C, Chen L, Ma C (2009) Thermal aggregation, amino acid composition and in vitro digestibility of vicilin-rich protein isolates from three Phaseolus legumes: a comparative study. Food Chem 113:957鈥?63View Article
  
• 作者单位：Luis Mojica (1)
  Elvira Gonz谩lez de Mej铆a (1)
  
  1. Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 228 ERML, MC-051, 1201 West Gregory Drive, Urbana, IL, 61801, USA
  
• 刊物主题：Food Science; Chemistry/Food Science, general; Nutrition; Ecology; Plant Physiology;
• 出版者：Springer US
• ISSN：1573-9104

文摘

Common bean (Phaseolus vulgaris L.) is a good source of protein, vitamins, minerals and complex carbohydrates. The objective was to compare protein profile, including anti-nutrient proteins, and potential bioactive peptides of improved common bean cultivars grown in Mexico and Brazil. Bean protein isolates (BPI) were prepared from 15 common bean cultivars and hydrolyzed using pepsin/pancreatin. Thirteen proteins were identified by SDS-PAGE and protein in-gel tryptic-digestion-LC/MS. Protein profile was similar among common bean cultivars with high concentrations of defense-related proteins. Major identified proteins were phaseolin, lectin, protease and 伪-amylase inhibitors. Lectin (159.2 to 357.9聽mg lectin/g BPI), Kunitz trypsin inhibitor (inh) (4.3 to 75.5聽mg trypsin inh/g BPI), Bowman-Birk inhibitor (5.4 to 14.3聽渭g trypsin-chymotrypsin inh/g BPI) and 伪-amylase inhibitor activity (2.5 to 14.9 % inhibition relative to acarbose/mg BPI) were higher in Mexican beans compared to Brazilian beans. Abundant peptides were identified by HPLC-MS/MS with molecular masses ranging from 300 to 1500聽Da and significant sequences were SGAM, DSSG, LLAH, YVAT, EPTE and KPKL. Potential bioactivities of sequenced peptides were angiotensin converting enzyme inhibitor (ACE), dipeptidyl peptidase IV inhibitor (DPP-IV) and antioxidant capacity. Peptides from common bean proteins presented potential biological activities related to control of hypertension and type-2 diabetes.