A rural worker infected with a bovine-prevalent genotype of Campylobacter fetus subsp. fetus supports zoonotic transmission and inconsistency of MLST and whole-genome typing

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• 作者：G. Iraola ; L. Betancor ; L. Calleros…
• 刊名：European Journal of Clinical Microbiology & Infectious Diseases
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：34
• 期：8
• 页码：1593-1596
• 全文大小：169 KB
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• 作者单位：G. Iraola (1) (2)
  L. Betancor (3)
  L. Calleros (2)
  P. Gadea (3)
  G. Algorta (3) (4)
  S. Galeano (5)
  P. Muxi (5)
  G. Greif (6)
  R. P茅rez (2)
  
  1. Unidad de Bioinform谩tica, Institut Pasteur de Montevideo, Montevideo, 11400, Uruguay
  2. Secci贸n Gen茅tica Evolutiva, Facultad de Ciencias, Universidad de la Rep煤blica, Montevideo, Uruguay
  3. Departamento de Bacteriolog铆a y Virolog铆a, Instituto de Higiene, Facultad de Medicina, Universidad de la Rep煤blica, Montevideo, Uruguay
  4. Laboratorio de Microbiolog铆a, Hospital Brit谩nico, Montevideo, Uruguay
  5. Unidad de Hematolog铆a, Hospital Brit谩nico, Montevideo, Uruguay
  6. Unidad de Biolog铆a Molecular, Institut Pasteur de Montevideo, Montevideo, Uruguay
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Internal Medicine
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1435-4373

文摘

Whole-genome characterisation in clinical microbiology enables to detect trends in infection dynamics and disease transmission. Here, we report a case of bacteraemia due to Campylobacter fetus subsp. fetus in a rural worker under cancer treatment that was diagnosed with cellulitis; the patient was treated with antibiotics and recovered. The routine typing methods were not able to identify the microorganism causing the infection, so it was further analysed by molecular methods and whole-genome sequencing. The multi-locus sequence typing (MLST) revealed the presence of the bovine-associated ST-4 genotype. Whole-genome comparisons with other C. fetus strains revealed an inconsistent phylogenetic position based on the core genome, discordant with previous ST-4 strains. To the best of our knowledge, this is the first C. fetus subsp. fetus carrying the ST-4 isolated from humans and represents a probable case of zoonotic transmission from cattle.