Assessment of yeast Saccharomyces cerevisiae component binding to Mycobacterium avium subspecies paratuberculosis using bovine epithelial cells
详细信息 查看全文
- 作者：Ziwei Li ; Qiumei You ; Faisury Ossa ; Philip Mead…
- 关键词：Saccharomyces cerevisiae ; Mycobacterium avium spp. paratuberculosis (MAP) ; Epithelial cell adhesion
- 刊名：BMC Veterinary Research
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：12
- 期：1
- 全文大小：1,837 KB
- 刊物主题：Veterinary Medicine; Zoology; Transgenics;
- 出版者：BioMed Central
- ISSN：1746-6148
文摘
Background Since yeast Saccharomyces cerevisiae and its components are being used for the prevention and treatment of enteric diseases in different species, they may also be useful for preventing Johne’s disease, a chronic inflammatory bowel disease of ruminants caused by Mycobacterium avium spp. paratuberculosis (MAP). This study aimed to identify potential yeast derivatives that may be used to help prevent MAP infection. The adherence of mCherry-labeled MAP to bovine mammary epithelial cell line (MAC-T cells) and bovine primary epithelial cells (BECs) co-cultured with yeast cell wall components (CWCs) from four different yeast strains (A, B, C and D) and two forms of dead yeast from strain A was investigated.