Modulation and the Underlying Mechanism of T Cells in Thymus of Mice by Oral Administration of Sodium Fluoride

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• 作者：Songna Yin ; Haibo Wu ; Chao Song ; Xin Chen ; Yong Zhang
• 关键词：Sodium fluoride ; Mice ; Thymus ; T cells ; TECs
• 刊名：Biological Trace Element Research
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：170
• 期：1
• 页码：194-200
• 全文大小：1,253 KB
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• 作者单位：Songna Yin (1) (2)
  Haibo Wu (1) (2)
  Chao Song (1) (2)
  Xin Chen (1) (2)
  Yong Zhang (1) (2)
  
  1. College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China
  2. Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, 712100, Shaanxi, China
  
• 刊物主题：Biochemistry, general; Biotechnology; Nutrition; Oncology;
• 出版者：Springer US
• ISSN：1559-0720

文摘

The underlying mechanism of thymic T cell regulation has been a hot topic of research in recent years. Fluoride is toxic at high concentrations and fluoride toxicity to thymic T cells was assessed in our study. To explore T cell responses to excess fluoride, different concentrations of fluoride were uptake by mice for 6 weeks. The expression of genes, including Foxn1, Cbx4, DLL4, and IL-7 gene, associated with the development and differentiation of T cells in thymic epithelial cells(TECs) was lower in the experimental groups than that in the control group. The percentages of CD4+ and CD8+ T cells that decreased with the fluoride administration were confirmed by flow cytometry. The mRNA levels of immunoregulatory cytokines IL-2 and IL-10, which participate in T cell proliferation, also declined in the experimental groups as compared with the control group. Expression of the T cell function-related genes CD2, PTPRC, CD69, and CD101, which are involved in thymic function in mice, decreased with the fluoride administration. Our findings suggest that the administration of high concentrations of fluoride to mice induces a decrease in CD4+ and CD8+ thymus T cells by harming TECs leading to the dysfunction of the thymus by altering the expression of T cell function-related genes and immunoregulatory cytokine production. Keywords Sodium fluoride Mice Thymus T cells TECs