Insight into substrate preference of two chimeric esterases by combining experiment and molecular simulation

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• 作者：Xiao-li Zhou (12353) (22353)
  Wei-wei Han (12353)
  Bai-song Zheng (12353)
  Yan Feng (12353) (22353)
  
• 关键词：Substrate preference ; Docking ; Chimeric enzyme ; Thermophilic esterase
• 刊名：Chemical Research in Chinese Universities
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：29
• 期：3
• 页码：533-537
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• 作者单位：Xiao-li Zhou (12353) (22353)
  Wei-wei Han (12353)
  Bai-song Zheng (12353)
  Yan Feng (12353) (22353)
  
  12353. Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, 130012, P. R. China
  22353. State Key Laboratory of Microbial Metabolism, College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai, 200240, P. R. China
  
• ISSN：2210-3171

文摘

Better understanding of the relationship between the substrate preference and structural module of esterases is helpful to novel enzyme development. For this purpose, two chimeric esterases AAM7 and PAR, constructed via domain swapping between two ancient thermophilic esterases, were investigated on their molecular simulation(including homology modeling, substrates docking and substrate binding affinity validation) and enzymatic assay(specific activities and activation energies calculating). Our results indicate that the factors contributing to the substrate preference of many enzymes especially the broad-specificity enzymes like esterases are multiple and complicated, the substrate binding domains or binding pockets are important but not the only factor for substrate preference.