Combined Urea Complexation and Argentated Silica Gel Column Chromatography for Concentration and Separation of PUFAs from Tuna Oil: Based on Improved DPA Level

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• 作者：Hongyan Mu ; Jun Jin ; Dan Xie ; Xiaoqiang Zou…
• 刊名：Journal of the American Oil Chemists' Society
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：93
• 期：8
• 页码：1157-1167
• 全文大小：539 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Industrial Chemistry and Chemical Engineering
  Analytical Chemistry
  Chemistry
  Biotechnology
  Biomaterials
  Agriculture
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1558-9331
• 卷排序：93

文摘

Eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid (DPA) isomers (22:5n-6 and 22:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) derived from tuna oil were concentrated by three stages of urea fractionation at various crystallization temperatures and different fatty acid/urea ratios. Thereafter, polyunsaturated fatty acids concentrate containing comparatively enriched DPA levels was purified by argentated silica gel column chromatography. A product containing 22.2 ± 0.6 % EPA, 4.6 ± 0.0 % DPAn-6, 5.9 ± 0.1 % DPAn-3 and 42.3 ± 1.2 % DHA was obtained at 1:1.6 fatty acid/urea ratio (w/w) by crystallization at −8 °C for 16 h, −20 °C for 8 h, and −8 °C for 16 h. A DPA isomer concentrate containing 26.1 ± 0.5 % DPAn-6 and 22.3 ± 0.4 % DPAn-3 was achieved by argentated silica gel chromatography in the 6 % acetone/n-hexane solvent fraction (v/v), and the recovery of both fatty acids was 66.1 ± 3.2 and 70.7 ± 2.2 %, respectively. Furthermore, 91.9 ± 2.5 % EPA and 99.5 ± 2.1 % DHA with recoveries of 47.8 ± 2.0 and 56.7 ± 3.3 %, respectively, were obtained in various fractions.KeywordsTuna oilUrea inclusionEicosapentaenoic acidDocosapentaenoic acidDocosahexaenoic acidArgentated silica gel column chromatography