Single amino acid replacement transforms mCherry to a far-red fluorescent protein

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• 作者：Yeji Kim ; Kyungju Song ; Hwajin Lee ; Dohyun Kim…
• 关键词：far ; red fluorescent proteins ; mCherry ; mutagenesis
• 刊名：Biotechnology and Bioprocess Engineering
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：21
• 期：6
• 页码：720-725
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology; Industrial and Production Engineering;
• 出版者：The Korean Society for Biotechnology and Bioengineering
• ISSN：1976-3816
• 卷排序：21

文摘

Far-red fluorescent proteins are beneficial for imaging in mammals. Here, starting from mCherry, the most commonly used among the different types of red fluorescent proteins (RFP), not having a H-bond network in its original form, we sought to recover the hydrogen bond network in mCherry. By comparing the structure of wtGFP and mCherry, we focused on a few key residues involved in a proton wire, and discovered an I197T mutant that showed a more red-shifted fluorescence. The detailed optical and photo-switching properties of related engineered RFPs are described. This study will guide further development of monomeric far-red FPs.