Effects of ivermectin on Danio rerio: a multiple endpoint approach: behaviour, weight and subcellular markers

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• 作者：I. Domingues ; R. Oliveira ; A. M. V. M. Soares ; M. J. B. Amorim
• 关键词：Weight variation ; Feeding behaviour ; Swimming behaviour ; Oxidative stress biomarkers ; Vitellogenin ; Veterinary pharmaceuticals
• 刊名：Ecotoxicology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：25
• 期：3
• 页码：491-499
• 全文大小：775 KB
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• 作者单位：I. Domingues (1) r> R. Oliveira (1) (2) r> A. M. V. M. Soares (1) r> M. J. B. Amorim (1) r>r>1. Departamento de Biologia & CESAM, Universidade de Aveiro, Campus de Santiago, CEP 3810-193, Aveiro, Portugal r> 2. Laboratório de Genética Toxicológica, Departamento de Genética e Morfologia, Instituto de Ciências Biológicas, Universidade de Brasília, Asa Norte, Brasília, DF, 70910-900, Brazil r>
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environmentr>Environmentr>Monitoring, Environmental Analysis and Environmental Ecotoxicologyr>Ecologyr>Environmental Managementr>
• 出版者：Springer Netherlands
• ISSN：1573-3017

文摘

Ivermectin (IVM) is a broad acting antihelmintic used in various veterinary pharmaceuticals. It has been shown that IVM enters the aquatic compartment and adversely affects organisms including fish. This study is based on the hypothesis that long term exposure to IVM affects fish and thus, the main objective was to assess the chronic effects of 0.25 and 25 µg IVM/L to zebrafish using multiple endpoints representative of several levels of biological organization: weight, behaviour (swimming and feeding) and subcellular markers including biomarkers for oestrogenicity (vitellogenin-VTG), oxidative stress (catalase-CAT and glutathione-S-transferase-GST) and neurotransmission (cholinesterase-ChE). Concentrations as low as 0.25 µg IVM/L disrupted the swimming behaviour, causing fish to spend more time at the bottom of aquaria. Such reduction of the swimming performance affected the feeding ability which is likely responsible for the weight loss. The effects on weight were gender differentiated, being more pronounced in males (0.25 µg IVM/L) than in females (25 µg IVM/L). Fish exposed to 25 µg/L exhibited darker coloration and mild curvature of the spine. No effects on VTG and AChE were observed, but a reduction on CAT and GST levels was observed in fish exposed to 25 µg IVM/L, although these alterations probably only reflect the general condition of the fish which was significantly compromised at this concentration. Despite that predicted environmental concentrations of IVM are below 0.25 µg/L, the behavioural effects may be translated into important ecological impacts, e.g. at predator–prey interactions where fish competitive advantage can be decreased. Future work should address the link between behaviour disruption and population fitness. The current study was based on a one experiment and multiple endpoint (anchored) approach, allowing the results to be integrated and linked towards a mechanistic understanding.