Hydrophobization of silk fibroin nanofibrous membranes by fluorocarbon plasma treatment to modulate cell adhesion and proliferation behavior

详细信息    查看全文

• 作者：Minyoung Lee (1)
  Young-Gwang Ko (1)
  Jae Baek Lee (1)
  Won Ho Park (2)
  Donghwan Cho (1)
  Oh Hyeong Kwon (1)
  
• 关键词：silk fibroin ; electrospinning ; nanofiber ; CF4 gas plasma treatment ; barrier membrane
• 刊名：Macromolecular Research
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：22
• 期：7
• 页码：746-752
• 全文大小：1,277 KB
• 参考文献：1. G. H. Altman, F. Diaz, C. Jakuba, T. Calabro, R. L. Horan, J. Chen, H. Lu, J. Richmond, and D. L. Kaplan, / Biomaterials, 24, 401 (2003). CrossRef
  2. M. Retzepi and N. Donos, / Clin. Oral Implants Res., 21, 567 (2010). CrossRef
  3. K.-H. Kim, L. Jeong, H.-N. Park, S.-Y. Shin, W.-H. Park, S.-C. Lee, T.-I. Kim, Y.-J. Park, Y.-J. Seol, Y.-M. Lee, Y. Ku, I.-C. Rhyu, S.-B. Han, and C.-P. Chung, / J. Biotechnol., 120, 327 (2005). CrossRef
  4. B.-M. Min, L. Jeong, K. Y. Lee, and W. H. Park, / Macromol. Biosci., 6, 285 (2006). CrossRef
  5. L. Jeong, K. Y. Lee, J. W. Liu, and W. H. Park, / Int. J. Biol. Macromol., 38, 140 (2006). CrossRef
  6. C. Zaharia, M.-R. Tudora, P. O. Stanescu, E. Vasile, and C. Cincu, / J. Optoelect. Adv. Mater., 14, 163 (2012).
  7. Y. Iriyama, T. Yasuda, D. L. Cho, and H. Yasuda, / J. Appl. Polym. Sci., 39, 249 (1990). CrossRef
  8. N. Hayashi, S. Ihara, S. Satoh, and C. Yamabe, / Rep. Fac. Sci. Engrg. Saga Univ., 30, No. 2 (2001).
  9. O. Kylián, M. Petr, A. Serov, P. Sola, O Polonskyi, J. Hanu?, A. Choukourov, and H. Biederman, / Vacuum, 100, 57 (2014). CrossRef
  10. B.-M. Min, G. Lee, S. H. Kim, Y. S. Nam, T. S. Lee, and W. H. Park, / Biomaterials, 25, 1289 (2004). CrossRef
  11. K. E. Park, S. Y. Jung, S. J. Lee, B.-M. Min, and W. H. Park, / Int. J. Biol. Macromol., 38, 165 (2006). CrossRef
  12. J. P. Chen, S.-H. Chen, and G.-J. Lai, / Nanoscale Res. Lett., 7, 1 (2012). CrossRef
  13. K. Yamada, Y. Tsuboi, and A. Itaya, / Thin Solid Films, 440, 208 (2003). CrossRef
  14. G. E. Wnek, M. E. Carr, D. G. Simpson, and G. L. Bowlin, / Nanoletters, 3, 213 (2003). CrossRef
  15. H. Fong, I. Chun, and D. H. Reneker, / Polymer, 40, 4585 (1999). CrossRef
  16. X. M. Mo, C. Y. Xu, M. Kotaki, and S. Ramakrishna, / Biomaterials, 25, 1883 (2004). CrossRef
  17. X. Zong, K. Kim, D. Fang, S. Ran, B. S. Hsiao, and B. Chu, / Polymer, 43, 4403 (2002). CrossRef
  18. H. Yoshimoto, Y. M. Shin, H. Terai, and J. P. Vacanti, / Biomaterials, 24, 2077 (2003). CrossRef
  19. I. S. Lee, O. H. Kwon, W. Meng, I. K. Kang, and Y. Ito, / Macromol. Res., 12, 374 (2004). CrossRef
  20. O. H. Kwon, I. S. Lee, Y.-G. Ko, W. Meng, K. H. Jung, I. K. Kang, and Y. Ito, / Biomed. Mater., 2, S52 (2007). CrossRef
  21. Y. Tamada and Y. Ikada. / J. Colloid Interface Sci., 155, 334 (1993). CrossRef
  22. Y. Tamada and Y. Ikada. / Polymer, 34, 2208 (1993). CrossRef
  23. Y. Tamada and Y. Ikada. / J. Biomed. Mater. Res., 28, 783 (1994). CrossRef
  
• 作者单位：Minyoung Lee (1)
  Young-Gwang Ko (1)
  Jae Baek Lee (1)
  Won Ho Park (2)
  Donghwan Cho (1)
  Oh Hyeong Kwon (1)
  
  1. Department of Polymer Science and Engineering, Kumoh National Institute of Technology, Gyeongbuk, 730-701, Korea
  2. Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon, 305-764, Korea
  
• ISSN：2092-7673

文摘

Saturated fluorocarbon (CF4) immobilized silk fibroin (SF) nanofibrous membranes were prepared and characterized for biomedical applications. Biocompatible barrier membranes that provide both hydrophobic and hydrophilic surface properties on each side are critical to prohibit soft tissue invasion into localized bone defect. As a barrier membrane, SF nanofibrous mat was fabricated by electrospinning method, and then subsequently modified with water vapor treatment for insolubilization in water and CF4 gas plasma treatment for surface hydrophobization. Morphology of SF nanofibrous mats were observed by scanning electron microscopy. Conformational change of insolubilized SF nanofibers was confirmed by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy and 13C nuclear magnetic resonance (NMR) spectroscopy. Immobilized fluorine atoms on CF4 plasma treated SF nanofibrous membranes were detected using electron spectroscopy for chemical analysis (ESCA). Water contact angle of the SF nanofiber membrane surface was analyzed by varying plasma input power and time. Insolubilized SF nanofibrous membrane maintained nonwoven mat structure without deformation after water immersion. SF nanofibrous membranes showed significant increment of water contact angle from 99.7° to 141.2° by CF4 gas plasma treatment. Fibroblasts on plasma untreated SF nanofibrous membranes were well attached and spread than a control tissue culture polystyrene dish. Fibroblasts on the CF4 gas plasma treated SF nanofibrous membrane showed significantly lower proliferation behavior than plasma untreated SF nanofibrous membranes. Fluorocarbon immobilized SF nanofibrous barrier membrane will be useful for biomedical applications such as a guided bone regeneration.