Decreased TK activity alters growth, yield and tolerance to low temperature and low light intensity in transgenic cucumber plants
详细信息 查看全文
- 作者：Huangai Bi ; Xubing Dong ; Guoxiu Wu ; Meiling Wang ; Xizhen Ai
- 关键词：Transketolase (TK) ; Net photosynthetic rate ; Antisense transgenic plants ; Chilling stress ; Cucumis sativus L
- 刊名：Plant Cell Reports
- 出版年：2015
- 出版时间：February 2015
- 年：2015
- 卷：34
- 期：2
- 页码：345-354
- 全文大小：752 KB
- 参考文献：1. Bi HG, Wang ML, Jiang ZS, Dong XB, Ai XZ (2011) Impacts of suboptimal temperature and low light intensity on the activities and gene expression of photosynthetic enzymes in cucumber seedling leaves. Chin J Appl Ecol 22:2894-900
2. Bi HG, Wang ML, Dong XB, Ai XZ (2013) Cloning and expression analysis of transketolase gene in / Cucumis sativus L. Plant Physiol Biochem 70:512-21 CrossRef
3. Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein using the principal of protein-dye binding. Anal Biochem 72:248-54 CrossRef
4. Chen JH, Gibson JL, McCueg LA, Tabita FR (1991) Identification, expression, and deduced primary structure of transketolase and other enzymes encoded within the form II CO2 fixation operon of / Rhodobacter sphaeroides. J Biol Chem 266:20447-0452
5. Flechner A, Dressen U, Westhoff P, Henze K, Schnarrenberger C, Martin W (1996) Molecular characterization of transketolase (EC 2.2. 1.1) active in the Calvin cycle of spinach chloroplasts. Plant Mol Biol 32:475-84 CrossRef
6. Frank RAW, Leeper FJ, Luisi BF (2007) Structure, mechanism and catalytic duality of thiamine-dependent enzymes. Cell Mol Life Sci 64:892-05 CrossRef
7. Haake VH, Zrenner R, Sonnewald U, Stitt M (1998) A moderate decrease of plastid aldolase activity inhibits photosynthesis, alters the levels of sugars and starch, and inhibits growth of potato plants. Plant J 14:147-57 CrossRef
8. Haake V, Geiger M, Walch-Liu P, Engels C, Zrenner R, Stitt M (1999) Changes in aldolase activity in wild-type potato plants are important for acclimation to growth irradiance and carbon dioxide concentration, because plastid aldolase exerts control over the ambient rate of photosynthesis across a range of growth conditions. Plant J 17:479-89 CrossRef
9. Henkes S, Sonnewald U, Badur R, Flachmann R, Stitt M (2001) A small decrease of plastid transketolase activity in antisense tobacco transformants has dramatic effects on photosynthesis and phenylpropanoid metabolism. Plant Cell 13:535-51 CrossRef
10. Hudson GS, Evans JR, von Caemmerer S, Arvidsson YBC, Andrews TJ (1992) Reduction of ribulose-1, 5-bisphosphate carboxylase/oxygenase content by antisense RNA reduces photosynthesis in transgenic tobacco plants. Plant Physiol 98:294-02 CrossRef
11. Jordan F (2003) Current mechanistic understanding of thiamin diphosphate-dependent enzymatic reactions. Nat Prod Rep 20:184-01 CrossRef
12. Kluger R (1987) Thiamin diphosphate: a mechanistic update on enzymic and nonenzymic catalysis of decarboxylation. Chem Rev 87:863-76 CrossRef
13. Kochetov G (2001) Functional flexibility of the transketolase molecule. Biochem (Moscow) 66:1077-085 CrossRef
14. Kovina MV, Kochetov GA (1998) Cooperativity and flexibility of active sites in homodimeric transketolase. FEBS Lett 440:81-4 CrossRef
15. Laemmli UK (1970) Cleavage of structural proteins during the assembly of head of bacteriophage T4. Nature 227:680-85 CrossRef
16. Metzger MH, Hollenberg CP (1994) Isolation and characterization of the / Pichia stipitis transketolase gene and expression in a xylose-utilising / Saccharomyces cerevisiae transformant. Appl Microbiol Biotechnol 42:319-25
17. Portis AR Jr, Parry MAJ (2007) Discoveries in Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase): a historical pers - 作者单位：Huangai Bi (1)
Xubing Dong (1)
Guoxiu Wu (1)
Meiling Wang (1)
Xizhen Ai (1)
1. State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong St., Tai’an, 271018, Shandong, People’s Republic of China - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Cell Biology
Plant Sciences
Biotechnology
Plant Biochemistry - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-203X
文摘
Key message Four CsTK antisense transgenic cucumber plants were obtained. Decreased TK activity decreased the photosynthetic rate, seed germination rate, growth yield, and the tolerance to low temperature and weak light stress. Abstract Transketolase (TK, EC 2.2.1.1) is a key enzyme in the photosynthetic carbon reduction cycle (Calvin cycle). A cDNA fragment (526?bp) encoding transketolase was cloned from cucumber plants (Cucumis sativa L. cv ‘Jinyou 3- by RT-PCR. The antisense expression [(PBI-CsTK(?] vector containing the CsTK gene fragment was constructed. The resulting plasmid was introduced into the cucumber inbred lines -8-1-using the agrobacterium-mediated method, and four antisense transgenic cucumber plants were obtained. Decreased CsTK expression either unaltered or slightly increased the mRNA abundance and activities of the other main enzymes in the Calvin cycle, however, it decreased the TK activity and net photosynthetic rate (Pn) in antisense transgenic cucumber leaves. Antisense plants showed decreases in the growth, ratio of female flowers and yield compared with the wild-type (WT) plants. The decrease in Pn, stomatal conductance (Gs), transpiration rate (Tr), photochemical efficiency (Fv/Fm) and actual photochemical efficiency of PSII (ΦPSII) and the increase in electrolyte leakage (EL) were greater in antisense transgenic plants than in WT plants under low temperature (5?°C) and low light intensity (100?μmol?m??s?).