Synthesis of bifunctional TiO2@SiO2-B(OH)2@Fe3O4@TiO2 sandwich-like nanosheets for sequential selective enrichment of phosphopeptides and glycopeptides for mass sp
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- 作者：Dongpo Xu ; Mingxia Gao ; Chunhui Deng…
- 关键词：Sandwich ; like nanosheet ; Boronic acid ; Titanium dioxide ; Glycopeptides ; Phosphopeptides ; MALDI ; TOF mass spectrometry
- 刊名：Analytical and Bioanalytical Chemistry
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：408
- 期：20
- 页码：5489-5497
- 全文大小：1,862 KB
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Mingxia Gao (1)
Chunhui Deng (1)
Xiangmin Zhang (1)
1. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, China - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Analytical Chemistry
Food Science
Inorganic Chemistry
Physical Chemistry
Monitoring, Environmental Analysis and Environmental Ecotoxicology - 出版者：Springer Berlin / Heidelberg
- ISSN：1618-2650
- 卷排序：408
文摘
In this work, the bifunctional TiO2@SiO2-B(OH)2@Fe3O4@TiO2 sandwich-like nanosheets were designed and synthesized for the sequential selective enrichment of phosphopeptides and glycopeptides. Due to the bifunctional property of the titanium dioxide and the boronic acid group, the nanosheets were successfully applied to the enrichment of phosphopeptides and glycopeptides sequentially, evaluated by capturing phosphopeptides from tryptic digestion of model phosphoprotein bovine β-casein diluted to 0.02 ng/μL (8 × 10−16 mol/μL) and glycopeptides from tryptic digestion of model glycoprotein horseradish peroxidase (HRP) diluted to 0.1 ng/μL (2.5 × 10−15 mol/μL). The enrichment selectivity of the bifunctional nanosheets was evaluated by capturing phosphopeptides from a peptide mixture of β-casein and bovine serum albumin (BSA) with the molar ratio of 1:1000 (8.3 × 10−12 mol of β-casein and 8.3 × 10−9 mol of BSA in 100 μL) and glycopeptides from a peptide mixture of HRP and BSA up to the ratio of 1:50 (5.0 × 10−11 mol of HRP and 2.5 × 10−9 mol of BSA in 100 μL).