Parkin promotes proteasomal degradation of p62: implication of selective vulnerability of neuronal cells in the pathogenesis of Parkinson’s disease

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• 作者：Pingping Song ; Shanshan Li ; Hao Wu ; Ruize Gao ; Guanhua Rao ; Dongmei Wang…
• 关键词：parkin ; sequestosome1/p62 ; ubiquitin ; substantia nigra
• 刊名：Protein & Cell
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：7
• 期：2
• 页码：114-129
• 全文大小：2,388 KB
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• 作者单位：Pingping Song (1)
  Shanshan Li (1)
  Hao Wu (2)
  Ruize Gao (1)
  Guanhua Rao (1)
  Dongmei Wang (3)
  Ziheng Chen (2)
  Biao Ma (1)
  Hongxia Wang (1)
  Nan Sui (3)
  Haiteng Deng (4)
  Zhuohua Zhang (5)
  Tieshan Tang (2)
  Zheng Tan (2)
  Zehan Han (6)
  Tieyuan Lu (6)
  Yushan Zhu (1)
  Quan Chen (1) (2)
  
  1. State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, 300071, China
  2. State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China
  3. Institute of Psychology, Chinese Academy of Sciences, Beijing, 100101, China
  4. College of Life Sciences, Tsinghua University, Beijing, 100084, China
  5. State Key Laboratory of Medical Genetics, Xiangya Medical School, Central South University, Changsha, 410078, China
  6. Department of Health and Sports Science, Tianjin University of Sport, Tianjin, 300381, China
  
• 刊物主题：Biochemistry, general; Protein Science; Cell Biology; Stem Cells; Human Genetics; Developmental Biology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1674-8018

文摘

Mutations or inactivation of parkin, an E3 ubiquitin ligase, are associated with familial form or sporadic Parkinson’s disease (PD), respectively, which manifested with the selective vulnerability of neuronal cells in substantia nigra (SN) and striatum (STR) regions. However, the underlying molecular mechanism linking parkin with the etiology of PD remains elusive. Here we report that p62, a critical regulator for protein quality control, inclusion body formation, selective autophagy and diverse signaling pathways, is a new substrate of parkin. P62 levels were increased in the SN and STR regions, but not in other brain regions in parkin knockout mice. Parkin directly interacts with and ubiquitinates p62 at the K13 to promote proteasomal degradation of p62 even in the absence of ATG5. Pathogenic mutations, knockdown of parkin or mutation of p62 at K13 prevented the degradation of p62. We further showed that parkin deficiency mice have pronounced loss of tyrosine hydroxylase positive neurons and have worse performance in motor test when treated with 6-hydroxydopamine hydrochloride in aged mice. These results suggest that, in addition to their critical role in regulating autophagy, p62 are subjected to parkin mediated proteasomal degradation and implicate that the dysregulation of parkin/p62 axis may involve in the selective vulnerability of neuronal cells during the onset of PD pathogenesis. Keywords parkin sequestosome1/p62 ubiquitin substantia nigra