Production of recombinant miraculin protein using transgenic citrus cell suspension culture system

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• 作者：Seong Beom Jin (1)
  Hyeon Jin Sun (2)
  Md Adnan Al Bachchu (3)
  Sung Jin Chung (2)
  Jongwoo Lee (2)
  Song-I Han (3)
  Jeong Hun Yun (3)
  Kyung Whan Boo (2)
  Dongsun Lee (3)
  Key Zung Riu (1) (2) (3)
  Jae-Hoon Kim (1) (2) (3)
  
• 关键词：Citrus callus ; Miraculin ; Recombinant protein ; Suspension cell culture
• 刊名：Journal of the Korean Society for Applied Biological Chemistry
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：56
• 期：3
• 页码：271-274
• 全文大小：348KB
• 参考文献：1. Bachchu MAA, Jin SB, Park JW, Boo KH, Sun HJ, Lee HY et al. (2011a) Agrobacterium-mediated transformation using embryogenic calli in Satsuma mandarin (Citrus unshiu Marc.) cv. Miyagawa wase. / Hort Environ Biotechnol 52, 170-75 CrossRef
  2. Bachchu MAA, Jin SB, Park JW, Boo KH, Sun HJ, Lee HY et al. (2011b) Functional expression of miraculin, a taste-modifying protein, in transgenic Miyagawa Wase Satsuma Mandarin (Citrus unshiu Marc.). / J Korean Soc Appl Biol Chem 54, 24-9 CrossRef
  3. Carimi F, Maria CT, Febio DP, and Francesco G (1998) Somatic embryogenesis and plant regeneration from undeveloped ovules and sitgma/style explants of sweet orange navel group [Citrus sinensis (L.) Osb.]. / Plant Cell, Tissue Organ Cult 54, 183-89 CrossRef
  4. Dutt M and Grosser JW (2010) An embryogenic suspension cell culture system for Agrobacterium-mediated transformation of citrus. / Plant Cell Rep 29, 1251-260 CrossRef
  5. Hirai T, Go F, Hideo K, Naoya F, and Hiroshi E (2010) Production of recombinant miraculin using transgenic tomatoes in a closed cultivation system. / J Agric Food Chem 58, 6096-101 CrossRef
  6. Hiwasa-Tanase K, Hirai T, Kato K, Duhita N, and Ezura H (2012) From miracle fruit to transgenic tomato: mass production of the tastemodifying protein miraculin in transgenic plants. / Plant Cell Rep 31, 513-25 CrossRef
  7. Huang TK and McDonald KA (2012) Bioreactor systems for in vitro production of foreign proteins using plant cell cultures. / Biotechnol Adv 30, 398-09 CrossRef
  8. Ito K, Sugawara T, Koizumi A, Nakajima K, Shimizu-Ibuka A, Shiroishi M et al. (2010) Bulky high-mannose-type N-glycan blocks the tastemodifying activity of miraculin. Biochim / Biophys Acta 1800, 986-92 CrossRef
  9. Jin SB, Song KJ, and Riu KZ (2007) Serveral factors affecting embryogeneic culture maintenance and shoot regeneration in ‘Miyagawqa Wase-satusuma mandarin (Citrus unshiu). / Hort Environ Biotechol 48, 167-70
  10. Kim NS, Yu HW, Chung ND, Shin YJ, Kwon TH, and Yang MS (2011) Production of functional recombinant bovine trypsin in transgenic rice cell suspension cultures. / Protein Expr Purif 76, 121-26 CrossRef
  11. Matsuyama T, Satoh M, Nakata R, Aoyama T, and Inoue H (2009) Functional expression of miraculin, a taste-modifying protein in Escherichia coli. / J Biochem 145, 445-50 CrossRef
  12. Sugaya T, Yano M, Sun HJ, Hirai T, and Ezura H (2008) Transgenic strawberry expressing the taste-modifying protein miraculin. / Plant Biotechnol 25, 329-33 CrossRef
  13. Sun HJ, Cui ML, Ma B, and Ezura H (2006) Functional expression of the taste-modifying protein, miraculin, in transgenic lettuce. / FEBS Lett 580, 620-26 CrossRef
  14. Sun HJ, Kataoka H, Yano M, and Ezura H (2007) Genetically stable expression of functional miraculin, a new type of alternative sweetener, in transgenic tomato plants. / Plant Biotech J 5, 768-77 CrossRef
  15. Theerasilp S and Kurihara Y (1988) Complete purification and characterization of the taste-modifying protein, miraculin, from miracle fruit. / J Biol Chem 263, 11536-1539
  
• 作者单位：Seong Beom Jin (1)
  Hyeon Jin Sun (2)
  Md Adnan Al Bachchu (3)
  Sung Jin Chung (2)
  Jongwoo Lee (2)
  Song-I Han (3)
  Jeong Hun Yun (3)
  Kyung Whan Boo (2)
  Dongsun Lee (3)
  Key Zung Riu (1) (2) (3)
  Jae-Hoon Kim (1) (2) (3)
  
  1. Subtropica/Tropical Plant Gene Bank, Jeju National University, Jeju, 690-756, Republic of Korea
  2. Subtropical Horticulture Research Institute, Jeju National University, Jeju, 690-756, Republic of Korea
  3. Faculty of Biotechnology, College of Applied Life Science, Jeju National University, SARI, Jeju, 690-756, Republic of Korea
  
• ISSN：2234-344X

文摘

Miraculin gene containing the N-terminal signal peptide was introduced into navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) callus cells by Agrobacterum-mediated transformation. Transgenic somatic embryos were screened on the shoot induction medium containing 25 mg hygromycin L?. Citrus callus cells were reproduced from the green color somatic embryos on the callus reproduction medium. The obtained transgenic cells were cultured in Murashige and Tucker’s liquid medium containing 50 g sucrose L? in a shaking incubator. Similar to the native miraculin, the secreted recombinant miraculin protein formed a disulfide-linked dimer and retained taste-modifying activity. The stability of recombinant protein expression was confirmed over nine generations of callus. This production system can be an excellent alternative for producing various recombinant proteins as well as miraculin.