Graphene oxide nanoparticles for two-photon fluorescence imaging of zebrafish

详细信息    查看全文

• 作者：Dongyu Li ; Li Xue ; Zhenfeng Zhu ; Xinyuan Zhao ; Jun Qian
• 关键词：Graphene oxide nanoparticles ; Two ; photon fluorescence imaging ; Zebrafish ; Biocompatible
• 刊名：Optical and Quantum Electronics
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：48
• 期：11
• 全文大小：2,501 KB
• 刊物主题：Optics, Optoelectronics, Plasmonics and Optical Devices; Electrical Engineering; Characterization and Evaluation of Materials; Computer Communication Networks;
• 出版者：Springer US
• ISSN：1572-817X
• 卷排序：48

文摘

Two-photon microscopy is an important technology for both ex vivo tissue and in vivo animal imaging, due to its deep-penetration and optical sectioning capability in biological tissue. Graphene has consistently been a hot research topic owing to its unique optical, electronic and mechanical properties. In this paper, we synthesized graphene oxide nanoparticles (GONs), which is a derivative of graphene, and characterized its nonlinear optical properties. GONs were microinjected into the embryos of zebrafish, and no in vivo toxicity towards zebrafish could be observed according to a series of biological testings. We then applied GONs in two-photon microscopy for bioimaging of zebrafish. The GONs treated zebrafish was imaged at different growth stages, with a scanning microscope equipped with a 720 nm-femtosecond laser. GONs generated strong two-photon fluorescence signals inside zebrafish, which could help characterizing the morphologies of zebrafish vividly.