Role of calcium content in antibacterial activity of donkeys-milk toward E. coli
详细信息 查看全文
- 作者：Ljubi?a ?. ?ari? (1)
Bojana M. ?ari? (1)
Anamarija I. Mandi? (1)
?arko S. Kevre?an (1)
Bojana B. Ikoni? (2)
Sne?ana ?. Kravi? (2)
Dubravka J. Jambrec (1) - 关键词：Donkeys-milk ; Antibacterial activity ; E. coli
- 刊名：European Food Research and Technology
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：239
- 期：6
- 页码：1031-1039
- 全文大小：264 KB
- 参考文献：1. Andrea C, Vincenzo C, Salvatore B, Anna MG, Rosaria S, Antonio Z et al (2009) Donkeys-milk protein fraction investigated by electrophoretic methods and mass spectrometric analysis. Int Dairy J 19:190-97 CrossRef
2. Bruhn O, Gr?tzinger J, Cascorbi I, Jung S (2011) Antimicrobial peptides and proteins of the horse e insights into a well-armed organism. Vet Res, 42: 98, (22 pages)
3. Christensen T, Gooden DM, Kung JE, Toone EJ (2003) Additivity and the physical basis of multivalency effects: a thermodynamic investigation of the calcium EDTA interaction. J Am Chem Soc 125:7357-366 CrossRef
4. Conte F, Scatassa ML, Monsu G, Lo Verde V, Finocchiaro A, De Fino M (2006) Monitoring of safety and quality of donkey’s milk. In: Smulders FJM (ed) Food safety assurance and veterinary public health: Towards a risk-based chain control, 1st edn. Wageningen Academic Publishers, Wageningen
5. Coppola R, Salimei E, Succi M, Sorrentino E, Nanni M, Ranieri P (2002) Behaviour of / Lactobacillus rhamnosus strains in ass’s milk. Ann Microbiol 52:55-0
6. Desmet J, Van Dael H, Van Cauwelaert F, Nitta K, Sugai S (1989) Comparison of the binding of Ca2+ and Mn2+ to bovine α-lactalbumin and equine lysozyme. J Inorg Biochem 37:185-91 CrossRef
7. EC (1995) Directive no 95/2/EC of the European Parliament and of the council of 20 February 1995 laying down directive on food additives other than colours and sweeteners (OJ No L 61, 18. 3. 1995, p. 1). http://ec.europa.eu/food/fs/sfp/addit_flavor/flav11_en.pdf Accessed 12.12.13
8. Etana M, Endeabu B, Jenberie S, Negussie H (2011) Determination of reference physiological values for working donkeys of Ethiopia. Ethiopian Vet J 15:79-6 CrossRef
9. Fantuz F, Ferraro S, Todini L, Piloni R, Mariani P, Salimei E (2012) Donkey milk concentration of calcium, phosphorus, potassium, sodium and magnesium. Int Dairy J 24:143-45 CrossRef
10. Farnaud S, Evans RW (2003) Lactoferrin-a multifunctional protein with antimicrobial properties. Mol Immunol 40:395-05 CrossRef
11. Floris R, Recio I, Berkhout B, Visser S (2003) Antibacterial and antiviral effects of milk proteins and derivatives thereof. Curr Pharm Des 9:1257-275 CrossRef
12. Fotadar U, Zaveloff P, Terracio L (2005) Growth of / Escherichia coli at elevated temperatures. J Basic Microb 45:403-04 CrossRef
13. Guo HY, Pang K, Zhang XY, Zhao L, Chen SW, Dong ML, Ren FZ (2007) Composition, physiochemical properties, nitrogen fraction distribution, and amino acid profile of donkey milk. J Dairy Sci 90:1635-643 CrossRef
14. Haezebrouck P, Noppe W, Van Dael H, Hanssens I (1992) Hydrophobic interaction of lysozyme and alpha-lactalbumin from equine milk whey. BBA-Protein Struct M 1122:305-10 CrossRef
15. Iacono G, Carroccio A, Cavataio F, Montalto G, Soresi M, Balsamo V (1992) Use of ass’s milk in multiple food allergy. J Pediatr Gastr Nutr 14:177-81 CrossRef
16. Ibrahim HR, Higashiguchi S, Koketsu M, Juneja LR, Kim M, Yamamoto T, Sugimoto Y, Aoki T (1996) Partially unfolded lysozyme at neutral pH agglutinates and kills Gram-negative and Gram-positive bacteria through membrane damage mechanism. J Agr Food Chem 44:3799-806 CrossRef
17. ISO (2001) Microbiology of food and animal feeding stuffs -Horizontal method for the enumeration of beta-glucuronidase-positive / Escherichia coli -Par - 作者单位：Ljubi?a ?. ?ari? (1)
Bojana M. ?ari? (1)
Anamarija I. Mandi? (1)
?arko S. Kevre?an (1)
Bojana B. Ikoni? (2)
Sne?ana ?. Kravi? (2)
Dubravka J. Jambrec (1)
1. Institute of Food Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000, Novi Sad, Serbia
2. Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000, Novi Sad, Serbia - ISSN：1438-2385
文摘
The objective of this study was to investigate the effect of calcium content in raw donkeys-milk (DM) on its antibacterial activity against Escherichia coli. Antibacterial assay was performed in artificially contaminated milk samples (without and with added CaCl2 and EDTA) incubated at 38?°C (donkeys-body temperature). The EDTA was added to DM to bind its calcium ions, while CaCl2 was used as the donor of calcium ions. The content of calcium, lysozyme (LYZ) and lactoferrin (LF) as well as pH value were determined in all of the tested milk samples. DM samples showed varying degrees of antibacterial activity against the tested E. coli strains. The milk samples with higher calcium content possessed stronger antibacterial potential toward the tested bacteria. The determinated calcium-dependant antibacterial activity of DM was proved thorough addition of CaCl2 and EDTA to DM. Calcium content in tested samples was in the range from 377.1 to 1,231?mg/l. The LYZ and LF in tested DM were present at concentrations between 0.98-.74?g/l and 2.2-4.3?mg/l, respectively. LF was not detected in five of the tested samples, and pH values of the samples were in range from 7.11 to 7.25.