Identification of glycosylated regions in pneumococcal PspA conjugated to serotype 6B capsular polysaccharide

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• 作者：Giovana Cappio Barazzone (1)
  Vittoria Pinto (2)
  Danilo Donnarumma (2)
  Martha Massako Tanizaki (1)
  Nathalie Norais (2)
  Francesco Berti (2)
  
• 关键词：Conjugate vaccines ; Streptococcus pneumoniae serotype 6B ; PspA ; Mass spectrometry
• 刊名：Glycoconjugate Journal
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：31
• 期：3
• 页码：259-269
• 全文大小：803 KB
• 参考文献：1. O鈥橞rien, K.L., Wolfson, L.J., Watt, J.P., Henkle, E., Deloria-Knoll, M., McCall, N., Lee, E., Mulholland, K., Levine, O.S., Cherian, T.: Burden of disease caused by / Streptococcus pneumoniae in children younger than 5 years: global estimates. Lancet 374, 893鈥?02 (2009) CrossRef
  2. Pilishvili, T., Lexau, C., Farley, M.M., Hadler, J., Harrison, L.H., Bennett, N.M., Reingold, A., Thomas, A., Schaffner, W., Craig, A.S., Smith, P.J., Beall, B.W., Whitney, C.G., Moore, M.R.: Sustained reductions in invasive pneumococcal disease in the era of conjugate vaccine. J Infect Dis 201, 32鈥?1 (2010) CrossRef
  3. Lynch 3rd, J.P., Zhanel, G.G.: / Streptococcus pneumoniae: epidemiology and risk factors, evolution of antimicrobial resistance, and impact of vaccines. Curr Opin Pulm Med 16, 217鈥?25 (2010)
  4. Mehr, S., Wood, N.: / Streptococcus pneumoniae鈥?/em>a review of carriage, infection, serotype replacement and vaccination. Paediatr Respir Rev 13, 258鈥?64 (2012) CrossRef
  5. Crain, M.J., Waltman II, W.D., Turner, J.S., Yother, J., Talkington, D.F., McDaniel, L.S., Gray, B.M., Briles, D.E.: Pneumococcal surface protein A (PspA) is serologically highly variable and is expressed by all clinically important capsular serotypes of / Streptococcus pneumoniae. Infect Immun 58, 3293鈥?299 (1990)
  6. Waltman, W.D., Mcdaniel, L.S., Gray, B.M., Briles, D.E.: Variation in the molecular weight of Pspa (pneumococcal surface protein A) among / Streptococcus pneumoniae. Microb Pathog 8, 61鈥?9 (1990) CrossRef
  7. Yother, J., Briles, D.E.: Structural properties and evolutionary relationships of Pspa, a surface protein of / Streptococcus pneumoniae, as revealed by sequence analysis. J Bacteriol 174, 601鈥?09 (1992)
  8. Hollingshead, S.K., Becker, R., Briles, D.E.: Diversity of PspA: mosaic genes and evidence for past recombination in / Streptococcus pneumoniae. Infect Immun 68, 5889鈥?900 (2000) CrossRef
  9. Hollingshead, S.K., Baril, L., Ferro, S., King, J., Coan, P., Briles, D.E.: Pneumococcal surface protein A (PspA) family distribution among clinical isolates from adults over 50 years of age collected in seven countries. J Med Microbiol 55, 215鈥?21 (2006) CrossRef
  10. Brandileone, M.C., Andrade, A.L., Teles, E.M., Zanella, R.C., Yara, T.I., Di Fabio, J.L., Hollingshead, S.K.: Typing of pneumococcal surface protein A (PspA) in / Streptococcus pneumoniae isolated during epidemiological surveillance in Brazil: towards novel pneumococcal protein vaccines. Vaccine 22, 3890鈥?896 (2004) CrossRef
  11. Senkovich, O., Cook, W.J., Mirza, S., Hollingshead, S.K., Protasevich, I.I., Briles, D.E., Chattopadhyay, D.: Structure of a complex of human lactoferrin N-lobe with pneumococcal surface protein a provides insight into microbial defense mechanism. J Mol Biol 370, 701鈥?13 (2007) CrossRef
  12. Tu, A.H.T., Fulgham, R.L., McCrory, M.A., Briles, D.E., Szalai, A.J.: Pneumococcal surface protein A inhibits complement activation by / Streptococcus pneumoniae. Infect Immun 67, 4720鈥?724 (1999)
  13. Ren, B., Szalai, A.J., Hollingshead, S.K., Briles, D.E.: Effects of PspA and antibodies to PspA on activation and deposition of complement on the pneumococcal surface. Infect Immun 72, 114鈥?22 (2004) CrossRef
  14. Csordas, F.C.L., Perciani, C.T., Darrieux, M., Goncalves, V.M., Cabrera-Crespo, J., Takagi, M., Sbrogio-Almeida, M.E., Leite, L.C.C., Tanizaki, M.M.: Protection induced by pneumococcal surface protein A (PspA) is enhanced by conjugation to a / Streptococcus pneumoniae capsular polysaccharide. Vaccine 26, 2925鈥?929 (2008) CrossRef
  15. Santamaria, R., Goulart, C., Perciani, C.T., Barazzone, G.C., Carvalho, R.J., Goncalves, V.M., Leite, L.C.C., Tanizaki, M.M.: Humoral immune response of a pneumococcal conjugate vaccine: capsular polysaccharide serotype 14-Lysine modified PspA. Vaccine 29, 8689鈥?695 (2011) CrossRef
  16. Perciani, C.T., Barazzone, G.C., Goulart, C., Carvalho, E., Cabrera-Crespo, J., Goncalves, V.M., Leite, L.C.C., Tanizaki, M.M.: Conjugation of polysaccharide 6B from / Streptococcus pneumoniae with pneumococcal surface protein A: PspA conformation and its effect on the immune response. Clin Vaccine Immunol 20, 858鈥?66 (2013) CrossRef
  17. Carvalho, R.J., Cabrera-Crespo, J., Tanizaki, M.M., Goncalves, V.M.: Development of production and purification processes of recombinant fragment of pneumococcal surface protein A in / Escherichia coli using different carbon sources and chromatography sequences. Appl Microbiol Biotechnol 94, 683鈥?94 (2012) CrossRef
  18. Bardotti, A., Averani, G., Berti, F., Berti, S., Carinci, V., D鈥橝scenzi, S., Fabbri, B., Giannini, S., Giannozzi, A., Magagnoli, C., Proietti, D., Norelli, F., Rappuoli, R., Ricci, S., Costantino, P.: Physicochemical characterisation of glycoconjugate vaccines for prevention of meningococcal diseases. Vaccine 26, 2284鈥?296 (2008) CrossRef
  19. Zon, G., Szu, S.C., Egan, W., Robbins, J.D., Robbins, J.B.: Hydrolytic stability of pneumococcal group 6 (type 6A and type 6B) capsular polysaccharides. Infect Immun 37, 89鈥?03 (1982)
  20. van Dam, J.E.G., Breg, J., Komen, R., Kamerling, J.P., Vliegenthart, J.F.G.: Isolation and structural studies of phosphate-containing oligosaccharides from alkaline and acid hydrolysates of / Streptococcus pneumoniae type 6B capsular polysaccharide. Carbohydr Res 187, 267鈥?86 (1989) CrossRef
  21. Roche, H., Hakansson, A., Hollingshead, S.K., Briles, D.E.: Regions of PspA/EF3296 best able to elicit protection against / Streptococcus pneumoniae in a murine infection model. Infect Immun 71, 1033鈥?041 (2003) CrossRef
  22. Garrity, R.R., Rimmelzwaan, G., Minassian, A., Tsai, W.P., Lin, G., de Jong, J.J., Goudsmit, J., Nara, P.L.: Refocusing neutralizing antibody response by targeted dampening of an lmmunodominant epitope. J Immunol 159, 279鈥?89 (1997)
  
• 作者单位：Giovana Cappio Barazzone (1)
  Vittoria Pinto (2)
  Danilo Donnarumma (2)
  Martha Massako Tanizaki (1)
  Nathalie Norais (2)
  Francesco Berti (2)
  
  1. Centro de Biotecnologia, Instituto Butantan, Avenida Dr. Vital Brazil, 1500, S茫o Paulo, Brazil, 05503-900
  2. Novartis Vaccines and Diagnostics, Via Fiorentina 1, Siena, Italy
  
• ISSN：1573-4986

文摘

Conjugate vaccines are being widely used since their introduction. Nowadays the interest in these vaccines is still growing and new antigens and conjugate chemistry are being studied and developed. Pneumococcal surface protein A (PspA) is one of the most studied pneumococcal antigens and is an important vaccine candidate. One approach to broaden the conjugate vaccine coverage could be the conjugation of the polysaccharide to a pneumococcal protein such as PspA. Previous results have shown that conjugated recombinant fragment of PspA (rPspA) not only maintained but also in some conjugates improved the induction of protective antibodies raised against the protein carrier. We describe here a characterization study to identify the domains of Streptococcus pneumoniae recombinant PspA (rPspA), from family 1 clade 1 and family 2 clade 3, involved in the conjugation with serotype 6B capsular polysaccharide.