Metabolic versatility of haloalkaliphilic bacteria from soda lakes belonging to the Alkalispirillum–Alkalilimnicola group

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• 作者：Dimitry Y. Sorokin ; Tatjana N. Zhilina ; Anatoly M. Lysenko ; Tatjana P. Tourova and Elizaveta M. Spiridonova
• 关键词：Alkalispirillum ; Alkalilimnicola ; Soda lakes ; Haloalkaliphilic denitrification
• 刊名：Extremophiles
• 出版年：2006
• 出版时间：June 2006
• 年：2006
• 卷：10
• 期：3
• 页码：213-220
• 全文大小：336 KB

文摘

Four new isolates were obtained from denitrifying enrichments with various electron donors using sediment samples from hypersaline soda lakes. Based on 16S rRNA gene analysis and DNA-DNA hybridization results, they were all identified as members of the Gammaproteobacteria closely associated with the Alkalispirillum–Alkalilimnicola group. Two isolates were obtained from samples enriched with nitrate as electron acceptor and H₂ or polysulfide as electron donors, and another two strains were obtained with N₂O as the electron acceptor and sulfide or acetate as electron donors. All four new isolates, together with the type strains of the genera Alkalispirillum and Alkalilimnicola originally described as obligate aerobes, were capable of anaerobic growth with acetate using either nitrate or N₂O as electron acceptors. Their denitrification pathway, however, was disrupted at the level of nitrite. RuBisCO form I gene was detected and sequenced in the new isolates and in Alkalilimnicola halodurans but not in Alkalispirillum mobile. These data, together with the evidence of Oremland et al. (Appl Environ Microbiol 68:4795–4802, 2002) on the potential of Alkalilimnicola sp. MLHE-1 for autotrophic growth with arsenite as electron donor and nitrate as electron acceptor, demonstrate much higher metabolic diversity of this specific group of haloalkaliphilic Gammaproteobacteria than was originally anticipated.