Neonatal estrogen treatment with β-estradiol 17-cypionate induces in post-pubertal mice inflammation in the ductuli efferentes, epididymis, and vas deferens, but not in the testis, provoking obstructive azoospermia

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• 作者：Munekazu Naito (1)
  Shuichi Hirai (1)
  Hayato Terayama (1)
  Ning Qu (1)
  Shogo Hayashi (2) (3)
  Naoyuki Hatayama (1)
  Hideto Kawamura (4)
  Takashi Nakano (2)
  Masahiro Itoh (1)
  
• 关键词：Estrogen ; Puberty ; Inflammation ; Epididymis ; Testis
• 刊名：Medical Molecular Morphology
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：47
• 期：1
• 页码：21-30
• 全文大小：7,621 KB
• 参考文献：1. Toppari J (2002) Environmental endocrine disrupters and disorders of sexual differentiation. Semin Reprod Med 20:305-12 CrossRef
  2. Toppari J (2008) Environmental endocrine disrupters. Sex Dev 2:260-67 CrossRef
  3. Delbès G, Levacher C, Habert R (2006) Estrogen effects on fetal and neonatal testicular development. Reproduction 132:527-38 CrossRef
  4. Nistal M, Paniagua R (1984) Inflammatory diseases of the epididymis and testis. In: Nistal M, Paniagua R (eds) Testicular and epididymal pathology. Thieme-Stratton, New York, pp 263-77
  5. Atanassova N, McKinnell C, Fisher J, Sharpe RM (2005) Neonatal treatment of rats with diethylstilboestrol (DES) induces stromal-epithelial abnormalities of the vas deferens and cauda epididymis in adulthood following delayed basal cell development. Reproduction 129:589-01 CrossRef
  6. Kawamura H, Nonogaki T, Yoshikawa K, Kimura M, Ichihara I, Nakano T (2000) Morphological changes in mouse accessory sex glands following neonatal estrogen treatment. Ann Anat 182:269-74 CrossRef
  7. Itoh M, Hiramine C, Hojo K (1991) A new murine model of autoimmune orchitis induced by immunization with viable syngeneic testicular germ cells alone. I. Immunological and histological studies. Clin Exp Immunol 83:137-42 CrossRef
  8. Arai Y, Suzuki Y, Nishizuka Y (1977) Hyperplastic and metaplastic lesions in the reproductive tract of male rats induced by neonatal treatment with diethylstilbestrol. Virchows Arch A Pathol Anat Histol 27:21-8 CrossRef
  9. Spearow JL, Barkley M (2001) Reassessment of models used to test xenobiotics for oestrogenic potency is overdue. Hum Reprod 16:1027-029 CrossRef
  10. Williams K, Saunders PT, Atanassova N, Fisher JS, Turner KJ, Millar MR, McKinnell C, Sharpe RM (2000) Induction of progesterone receptor immunoexpression in stromal tissue throughout the male reproductive tract after neonatal oestrogen treatment of rats. Mol Cell Endocrinol 164:117-31 CrossRef
  11. Woodham C, Birch L, Prins GS (2003) Neonatal estrogen down-regulates prostatic androgen receptor through a proteosome-mediated protein degradation pathway. Endocrinology 144:4841-850 CrossRef
  12. Ogiso T, Arai Y, Fukushima S, Nishizuka Y, Ito N (1982) Enhancing effect of diethylstilbestrol on urinary bladder carcinogenesis in neonatally castrated male rats. Gann 73:862-69
  13. Pylkk?nen L, Santti R, Newbold R, McLachlan JA (1991) Regional differences in the prostate of the neonatally estrogenized mouse. Prostate 18:117-29 CrossRef
  14. Nakai M, Uchida K, Teuscher C (1999) The development of male reproductive organ abnormalities after neonatal exposure to tamoxifen is genetically determined. J Androl 20:626-34
  15. Morgan AD (1976) Inflammation and infestation of the testis and paratesticular structures. In: Pugh RBC (ed) Pathology of the testis. Blackwell, Oxford, pp 79-38
  16. Itoh M, Miyamoto K, Satriotomo I, Takeuchi Y (1999) Spermatic granulomata are experimentally induced in epididymides of mice receiving high-dose testosterone implants. I. A light-microscopical study. J Androl 20:551-58
  17. Maj T, S Awek A, Kaleta-Kuratewicz K, Che Mo Ska-Soyta A (2011) 17β-Estradiol and interferon tau interact in the regulation of the immune response in a model of experimental autoimmune orchitis. J Interferon Cytokine Res 31:825-37 CrossRef
  18. Jarow JP, Espeland MA, Lipshultz LI (1989) Evaluation of the azoospermic patient. J Urol 142:62-5
  19. Practice Committee of American Society for Reproductive Medicine in collaboration with Society for Male Reproduction and Urology (2008) The management of infertility due to obstructive azoospermia. Fertil Steril 90:121-24 CrossRef
  20. Matsuda T, Horii Y, Yoshida O (1994) Obstructive azoospermia of unknown origin: sites of obstruction and surgical outcomes. J Urol 151:1543-546
  21. Han Y, Feng HL, Sandlow JI, Haines CJ (2009) Comparing expression of progesterone and estrogen receptors in testicular tissue from men with obstructive and nonobstructive azoospermia. J Androl 30:127-33 CrossRef
  
• 作者单位：Munekazu Naito (1)
  Shuichi Hirai (1)
  Hayato Terayama (1)
  Ning Qu (1)
  Shogo Hayashi (2) (3)
  Naoyuki Hatayama (1)
  Hideto Kawamura (4)
  Takashi Nakano (2)
  Masahiro Itoh (1)
  
  1. Department of Anatomy, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan
  2. Department of Anatomy, Aichi Medical University, Nagakute, Aichi, Japan
  3. Medicinal Education Center, Department of Medicine, Aichi Medical University, Nagakute, Aichi, Japan
  4. Department of Medicine, Aichi Medical University, Nagakute, Aichi, Japan
  
• ISSN：1860-1499

文摘

Neonatal estrogen treatment (NET) induces morphological changes in male reproductive organs. NET with β-estradiol 17-cypionate is reported to induce inflammation with stromal-epithelial abnormalities in the prostate and seminal vesicles in post-pubertal mice. The aim of this study was to investigate the histopathology of the testis, ductuli efferentes, epididymis, and vas deferens in mice after NET with β-estradiol 17-cypionate. No morphological changes in these organs were observed until 4?weeks after NET. However, some inflammatory cells were found in epididymis and vas deferens 6?weeks after NET. Eight weeks after NET, inflammatory cells spread to the ductuli efferentes and inflammation was severe from 6 to 12?weeks after NET. Inflammatory cells were never seen in the whole testis, but cystic dilatation of the rete testes with spermatogenic disturbance was found around the mediastinum testis. Many inflammatory cells emigrated into the lumen of the epididymis, resulting in complete absence of spermatozoa in the vas deferens. Most of the inflammatory cells penetrating into the epithelial layers of epididymal ducts were neutrophils. These results indicate that in post-pubertal mice, NET with β-estradiol 17-cypionate induces inflammation in the ductuli efferentes, epididymis, and vas deferens, but not in the testis, provoking obstructive azoospermia.