Cis-existence of H3K27me3 and H3K36me2 in mouse embryonic stem cells revealed by specific ions of isobaric modification chromatogram
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- 作者：Hailei Mao ; Gang Han ; Longyong Xu ; Duming Zhu ; Hanqing Lin…
- 刊名：Stem Cell Research & Therapy
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：6
- 期：1
- 全文大小：2,028 KB
- 刊物主题：Stem Cells; Cell Biology;
- 出版者：BioMed Central
- ISSN：1757-6512
文摘
Introduction Histone H3 lysine 27 trimethylation (H3K27me3) and H3 lysine 36 trimethylation (H3K36me3) are important epigenetic modifications correlated with transcription repression and activation, respectively. These two opposing modifications rarely co-exist in the same H3 polypeptide. However, a small but significant amount of H3 tails are modified with 5 methyl groups on K27 and K36 in mouse embryonic stem cells (mESCs) and it is unclear how the trimethylation is distributed on K27 or K36.