Duplex real-time PCR for the determination of wasabi (Eutrema wasabi) contents in horseradish (Armoracia rusticana) products applying the ΔΔct-method

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• 作者：René Köppel ; Thomas B. Bucher
• 刊名：European Food Research and Technology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：242
• 期：7
• 页码：1111-1115
• 全文大小：421 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Analytical Chemistry
  Biotechnology
  Agriculture
  Forestry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1438-2385
• 卷排序：242

文摘

Many products claim to contain wasabi. Due to its high price, original wasabi (Eutrema wasabi) is often replaced by its cheaper close relative horseradish (Armoracia rusticana). To analyze such products, we developed a duplex real-time PCR method determining the DNA content of wasabi and horseradish, applying the ΔΔct-method. The developed horseradish-specific system turned out to be highly specific within the plant family Brassicaceae. We validated this duplex real-time PCR system and present results from common commercial products. The system proved to be reliable for uncovering fraudulent practices in the wasabi product group.KeywordsMultiplexReal-time quantitative PCRWasabiHorseradishEutrema wasabiArmoracia rusticana