Improvement of sensitivity for Mycobacterium avium subsp. paratuberculosis (MAP) detection in bovine fecal samples by specific duplex F57/IC real-time and conventional
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- 作者：Ahmad Fawzy ; Tobias Eisenberg ; Amr El-Sayed…
- 关键词：Mycobacterium avium subsp. paratuberculosis (MAP) ; IS900 PCR ; Duplex real ; time PCR ; Enrichment ; Culture ; Detection limit
- 刊名：Tropical Animal Health and Production
- 出版年：2015
- 出版时间：April 2015
- 年：2015
- 卷：47
- 期：4
- 页码：721-726
- 全文大小：160 KB
- 参考文献：1. Bull, TJ, McMinn, EJ, Sidi-Boumedine, K, Skull, A, Durkin, D, Neild, P, Rhodes, G, Pickup, R, Hermon-Taylor, J (2003) Detection and verification of Mycobacterium avium subsp. paratuberculosis in fresh ileocolonic mucosal biopsy specimens from individuals with and without Crohn’s disease. Journal of Clinical Microbiology 41: pp. 2915-2923 CrossRef
2. Clark, DL, Koziczkowski, JJ, Radcliff, RP, Clarson, RA, Ellingson, JLE (2007) Detection of Mycobacterium avium subsp. paratuberculosis: comparing fecal culture versus serum enzyme-linked immunosorbent assay and direct fecal polymerase chain reaction. Journal of Dairy Science 91: pp. 2620-2627 CrossRef
3. Cousins, DV, Whittington, R, Marsh, I, Masters, A, Evans, RJ, Kluver, P (1999) Mycobacteria distinct from Mycobacterium avium subsp. paratuberculosis isolated from the feces of ruminants possess IS900-like sequences detectable IS900 polymerase chain reaction: implications for diagnosis. Molecular and Cellular Probes 13: pp. 431-442 CrossRef
4. Juan, L, Alvarez, J, Romero, B, Bezos, J, Castellanos, E, Aranaz, A, Mateos, A, Dominguez, L (2006) Comparison of four different culture media for isolation and growth of type II and type I/III Mycobacterium avium subsp. paratuberculosis strains isolated from cattle and goats. Applied and Environmental Microbiology 72: pp. 5927-5932 CrossRef
5. Dreier, S, Khol, JL, Stein, B, Fuchs, K, Guetler, S, Baumgartner, W (2006) Serological, bacteriological and molecular biological survey of paratuberculosis (Johne’s disease) in Austrian cattle. Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health 53: pp. 477-481 CrossRef
6. Eisenberg, T, Wolter, W, Lenz, M, Schlez, K, Zsch?ck, M (2013) Boot swabs to collect environmental samples from common locations in dairy herds for Mycobacterium avium ssp. paratuberculosis (MAP) detection. Journal of Dairy Research 80: pp. 485-489 CrossRef
7. Fernandez-Silva, JA, Correa-Valencia, NM, Ramirez, NF (2014) Systematic review of the prevalence of paratuberculosis in cattle, sheep, and goats in Latin America and the Caribbean. Tropical Animal Health and Production 46: pp. 1321-1340 CrossRef
8. Hoffmann, B, Depner, K, Schirrmeier, H, Beer, M (2006) A universal heterologous internal control system for duplex realtime RT-PCR assays used in a detection system for pestiviruses. Journal of Virological Methods 136: pp. 200-209 CrossRef
9. Hughes, VM, Stevenson, K, Sharp, JM (2001) Improved preparation of high molecular weight DNA for pulsed-field gel electrophoresis from mycobacteria. Journal of Microbiological Methods 44: pp. 209-215 CrossRef
10. Kralik, P, Slana, I, Kralova, A, Babak, V, Whitlock, RH, Pavlik, I (2011) Development of a predictive model for detection of Mycobacterium avium subsp. paratuberculosis in faeces by quantitative real time PCR. Veterinary Microbiology 149: pp. 133-138 CrossRef
11. Kralik, P, Beran, V, Pavlik, I (2012) Enumeration of Mycobacterium avium subsp. paratuberculosis by quantitative realtime PCR, culture on solid media and optical densitometry. BMC Research Notes 5: pp. 114 CrossRef
12. Losinger, WC (2005) Economic impact of reduced milk production associated with Johne’s disease on dairy operations in the USA. Journal of Dairy Research 72: pp. 425-432 CrossRef
13. Norton, S, Johnson, WO, Jones, G, Heuer, C (2010) Evaluation of diagnostic tests for Johne’s disease (Mycobacterium avium subsp. paratuberculosis) in New Zealand dairy cows. - 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Veterinary medicine
Zoology - 出版者：Springer Netherlands
- ISSN：1573-7438
文摘
Mycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of Johne’s disease in ruminants and a probable pathogen of Crohn’s disease in humans. Accurate, cost-effective, and time-relevant diagnostics are the basis for efficient control programs. This study was conducted as an attempt to re-evaluate MAP detection improvement by coupling solid media enrichment to a more specific IS900 conventional PCR and a very specific F57/IC real-time PCR. In a spiking experiment, we investigated the improvement of molecular-based MAP detection in feces after a culture-based enrichment step into Herrold’s egg yolk media with mycobactin J (HEYM-MJ) for different time intervals, when compared to traditional culture. Detection limit of culture was 0.33?×-04?bacteria?×?g? (33?CFU?g?), while that of IS900 PCR when coupled with an enrichment step for 2, 4, and 6?weeks was 0.33?×-05 (0.33?×-03?CFU?g?), 0.33?×-04 (33?CFU?g?), and 33 (>3.3?CFU?g?) bacteria?×?g?, respectively. Whereas the detection limits of F57/IC real-time PCR after the enrichment step for the same time intervals were 0.33?×-05 (0.33?×-03?CFU?g?), 0.33?×-03 (3.3?CFU?g?), and 33 (>3.3?CFU?g?) bacteria?×?g?, respectively. Altogether, enrichment of bovine fecal samples into solid media increased the sensitivity of specific molecular detection of MAP using IS900 conventional PCR and duplex F57/IC real-time PCR and offers an expedited and accurate alternative for MAP detection in bovine feces. Validation of these results is further recommended using field bovine fecal samples.