Express detection of nonylphenol in water samples by fluorescence polarization immunoassay

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• 作者：JuliaN. Yakovleva ; AnnaYu. Lobanova ; EkaterinaA. Shutaleva ; MariaA. Kourkina ; AndreyA. Mart’ianov ; AnatolyV. Zherdev ; BorisB. Dzantiev and SergeiA. Eremin
• 关键词：Nonylphenol ; Endocrine ; disrupting chemicals ; Antibody production ; Fluorescence polarization immunoassay ; Cross ; reactivity
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2004
• 出版时间：February 2004
• 年：2004
• 卷：378
• 期：3
• 页码：634-641
• 全文大小：294 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

The development of express method for detection of endocrine-disrupting chemicals (EDC) such as alkylphenols is required for ecological monitoring. Several attempts have been made to produce antibodies against 4-nonylphenol (NP) in recent years. This work describes the production of new antibodies against NP and also summarizes the characterization of antibodies obtained earlier. Three approaches used to produce alkylphenol-specific antibodies are compared; these are based on:1. -(4-hydroxyphenyl)nonanoic or -(4-hydroxyphenyl)heptanoic acid NP derivatives designed to mimic the linear NP isomer; 2. 4-aminophenol, which potentially mimics various substituted phenolic compounds with different side-chain structures at position 4 of the benzene ring; and 3. a mixture of branched NP isomers, conjugated to the carrier protein via a benzene ring by the Mannich reaction, and expected to be the closest mimic of NP structure by preserving its natural alkyl moiety. Fluorescence polarization immunoassays based on different combinations of antibody and labeled antigen for screening detection of NP were developed and structural aspects of assay sensitivity and specificity were investigated. The assays based on the antisera raised against -(4-hydroxyphenyl)nonanoic acid and NP conjugate via Mannich reaction are capable of express detection of NP with detection limit of 7 g mL–1 and assay dynamic range of 18–300 g mL–1.