刊名:Journal of Otolaryngology - Head & Neck Surgery
出版年:2017
出版时间:December 2017
年:2017
卷:46
期:1
全文大小:447KB
刊物主题:Otorhinolaryngology; Head and Neck Surgery; Plastic Surgery; Oral and Maxillofacial Surgery;
出版者:BioMed Central
ISSN:1916-0216
卷排序:46
文摘
BackgroundThe incidence of oropharyngeal squamous cell carcinoma (OPSCC) caused by oncogenic human papillomavirus (HPV) is rising worldwide. HPV-OPSCC is commonly diagnosed by RT-qPCR of HPV E6 and E7 oncoproteins or by p16 immunohistochemistry (IHC). Droplet digital PCR (ddPCR) has been recently reported as an ultra-sensitive and highly precise method of nucleic acid quantification for biomarker analysis. To validate the use of a minimally invasive assay for detection of oncogenic HPV based on oropharyngeal swabs using ddPCR. Secondary objectives were to compare the accuracy of ddPCR swabs to fresh tissue p16 IHC and RT-qPCR, and to compare the cost of ddPCR with p16 IHC.