Cr(VI) reduction in a chromate-resistant strain of Candida maltosa isolated from the leather industry

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• 作者：Ramí ; rez Ramí ; rez ; Rocí ; o ; Calvo M&eacute ; ndez ; Carlos ; Á ; vila Rodrí ; guez ; Mario ; Lappe ; Patricia ; Ulloa ; Miguel ; Vá ; zquez Juá ; rez ; Ricardo ; F&eacute ; lix Guti&eacute ; rrez
• 关键词：Candida maltosa ; Chromate ; reduction ; Chromate ; resistance ; Chromium
• 刊名：Antonie van Leeuwenhoek
• 出版年：2004
• 出版时间：2004
• 年：2004
• 卷：85
• 期：1
• 页码：63-68
• 全文大小：1.46 MB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Medical Microbiology
  Plant Sciences
  Soil Science and Conservation
  
• 出版者：Springer Netherlands
• ISSN：1572-9699

文摘

A Cr(VI)-resistant yeast was isolated from tanning liquors from a leather factory in Leon, Guanajuato, Mexico. Based on morphological and physiological analyses and the D1/D2 domain sequence of the 26S rDNA, the yeast was identified as Candida maltosa. Resistance of the strain to high Cr(VI) concentrations and its ability to chemically reduce chromium was studied. When compared to the three laboratory yeasts Candida albicans, Saccharomyces cerevisiae and Yarrowia lipolytica, the C. maltosa strain was found to tolerate chromate concentrations as high as 100 μg/ml. In addition to this phenotypic trait, the C. maltosa strain showed ability to reduce Cr(VI). Chromate reduction occurred both in intact cells (grown in culture medium or in soil containing chromate) as well as in cell-free extracts. NADH-dependent chromate reductase activity was found associated with soluble protein and, to a lesser extent, with the membrane fraction.