Immunohistochemical expression of retinoblastoma and phospho-retinoblastoma protein in lung sheep exposed to fluoro-edenite fibers

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• 作者：Giuseppe Musumeci (1)
  Carla Loreto (1)
  Venera Cardile (2)
  Maria Luisa Carnazza (1)
  Giuseppina Martinez (1)
  
• 关键词：Rb ; pRb ; Fluoro ; edenite fibers ; Lung ; In?vivo experiment
• 刊名：Anatomical Science International
• 出版年：2010
• 出版时间：June 2010
• 年：2010
• 卷：85
• 期：2
• 页码：74-78
• 全文大小：582KB
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• 作者单位：Giuseppe Musumeci (1)
  Carla Loreto (1)
  Venera Cardile (2)
  Maria Luisa Carnazza (1)
  Giuseppina Martinez (1)
  
  1. Department of Anatomy, Diagnostic Pathology, Forensic Medicine, Hygiene and Public Health, University of Catania, Via S. Sofia 87, 95123, Catania, Italy
  2. Department of Physiological Sciences, University of Catania, Via Andrea Doria 6, 95123, Catania, Italy
  

文摘

The immunohistochemical expression of retinoblastoma protein (Rb) and its phosphorylated form (pRb) was investigated in an in?vivo sheep model, i.e., lung tissue exposed to asbestos-like fibers. Rb is a phosphoprotein with a central role in controlling the cell cycle, cell growth regulation, differentiation, and apoptosis. pRb functions as a checkpoint in the G1 phase, favoring cell growth, while the hypophosphorylated form (Rb) not only promotes cell-cycle arrest and a return to the G1 phase, but also appears to be a key player in most instances of apoptosis. pRb overexpression was detected in alveolar epithelium and the interstitium, especially close to the fluoro-edenite fibers, whereas Rb immunostaining was faint or absent. The data suggest that the altered balance between pRb and Rb expression may be a programmed response to protect the organism against uncontrolled cell proliferation.