Development and Validation of Electromigration Technique for the Determination of Lincomycin and Clindamycin Residues in Poultry Tissues

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• 作者：Piotr Kowalski (1)
  Lucyna Konieczna (1)
  Ilona Ol?dzka (1)
  Alina Plenis (1)
  Tomasz B?czek (1)
  
• 关键词：Lincosamide antibiotics ; Micellar electrokinetic capillary chromatography (MEKC) ; Residue analysis ; Poultry tissues
• 刊名：Food Analytical Methods
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：7
• 期：2
• 页码：276-282
• 全文大小：186 KB
• 作者单位：Piotr Kowalski (1)
  Lucyna Konieczna (1)
  Ilona Ol?dzka (1)
  Alina Plenis (1)
  Tomasz B?czek (1)
  
  1. Department of Pharmaceutical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416, Gdańsk, Poland
  
• ISSN：1936-976X

文摘

An analytical method to quantify lincosamide residues (lincomycin and clindamycin) in poultry tissue samples by micellar electrokinetic capillary chromatography combined with UV detection has been developed and validated. The clean-up procedure with a simple deproteinization and solid-phase extraction was successfully applied for the determination of antibiotics in different edible tissue samples of poultry. The electrophoretic separation was achieved using an uncoated fused silica capillary (57?cm?×-5?μm i.d.), voltage 16?kV, temperature 22?°C, and the optimal wavelength at 200?nm. The running buffer consisting of 10?mM sodium tetraborate decahydrate (pH?9.3) and 25?mM sodium dodecylsulfate was employed. The validation of the method was performed by evaluation of the analytical parameters: linearity, specificity, precision, and accuracy with limits of detection of 13.2 and 18.5?ng/g, for clindamycin and lincomycin, respectively. Good intermediate precision was obtained with relative standard deviation values less than 12?%. The mean recoveries were 91.2 and 87.7?% for lincomycin and clindamycin, respectively. The obtained results confirm that the proposed method is capable to identify and quantify of lincosamide residues in animal tissues far below maximum residue limit values and could be suitable for routine application in commercial of poultry samples.