Replacing a suite of commercial pectinases with a single enzyme, pectate lyase B, in Saccharomyces cerevisiae fermentations of cull peaches
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- 作者：M. C. Edwards (1)
T. Williams (1)
S. Pattathil (2) (3)
M. G. Hahn (2) (3) (4)
J. Doran-Peterson (1) - 关键词：Saccharomyces cerevisiae ; PelB ; Glycome profile ; Ethanol
- 刊名：Journal of Industrial Microbiology and Biotechnology
- 出版年：2014
- 出版时间：April 2014
- 年：2014
- 卷：41
- 期：4
- 页码：679-686
- 全文大小：899 KB
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T. Williams (1)
S. Pattathil (2) (3)
M. G. Hahn (2) (3) (4)
J. Doran-Peterson (1)
1. Department of Microbiology, University of Georgia, Athens, GA, 30602, USA
2. Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA
3. BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA
4. Department of Plant Biology, University of Georgia, Athens, GA, 30602, USA - ISSN：1476-5535
文摘
Fermentation of pectin-rich biomass with low concentrations of polysaccharides requires some treatment of the pectin, but does not need complete degradation of the polysaccharide to reach maximum ethanol yields. Cull peaches, whole rotten fruits that are not suitable for sale, contain high concentrations of glucose (27.7?% dw) and fructose (29.3?% dw) and low amounts of cellulose (2.8?% dw), hemicellulose (4.5?% dw) and pectin (5.6?% dw). Amounts of commercial saccharification enzymes, cellulase and cellobiase can be significantly decreased and commercial pectinase mixtures can be replaced completely with a single enzyme, pectate lyase (PelB), while maintaining ethanol yields above 90?% of the theoretical maximum. PelB does not completely degrade pectin; it only releases short chain oligogalacturonides. However, the activity of PelB is sufficient for the fermentation process, and its addition to fermentations without commercial pectinase increases ethanol production by ~12?%.