Assessment and Implications of Intraspecific and Phenological Variability in Monoterpenes of Scots Pine (Pinus sylvestris) Foliage

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• 作者：Vera Thoss (1) (2) (4)
  Julianne O鈥橰eilly-Wapstra (3)
  Glenn R. Iason (1)
  
• 关键词：Absolute concentration ; 伪 ; pinene ; Camphene ; 尾 ; pinene ; 螖3 ; carene ; Chemotypes ; Relative composition ; Seasonal variation
• 刊名：Journal of Chemical Ecology
• 出版年：2007
• 出版时间：March 2007
• 年：2007
• 卷：33
• 期：3
• 页码：477-491
• 全文大小：455KB
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• 作者单位：Vera Thoss (1) (2) (4)
  Julianne O鈥橰eilly-Wapstra (3)
  Glenn R. Iason (1)
  
  1. The Macaulay Institute, Craigiebuckler, Aberdeen, AB15 8QH, UK
  2. School of Biological Sciences, Cruickshank Building, University of Aberdeen, Aberdeen, AB24 3UU, UK
  4. School of Chemistry, University of Wales, Bangor, LL57 2UW, UK
  3. School of Plant Science and CRC for Sustainable Production Forestry, University of Tasmania, Private Bag 55, Hobart, Tasmania, Australia, 7001
  

文摘

Scots pine populations contain individuals with widely differing amounts and composition of monoterpenes and exist as one of two chemotypes: with or without 螖3-carene. We investigated the significance for ecological studies of two types of variation in monoterpenes: (1) the inherent variability in the concentration of monoterpenes or their relative amounts in needles of seedlings, saplings, and mature trees; and (2) phenological variation in developing needles. The relative composition of needle monoterpenes in 5-year-old saplings changed during the needle development period until the final composition was reached upon needle maturity. Changes in composition depended on chemotype. Needles of the 鈥渘o-螖3-carene鈥?chemotype had higher absolute concentrations of 伪-pinene, 尾-pinene, camphene, and total monoterpenes than 鈥溛?sup class="a-plus-plus">3-carene鈥?chemotype. For the 鈥溛?sup class="a-plus-plus">3-carene鈥?chemotype, the relative concentration of 螖3-carene during the needle growing season and immediately after emergence of seedlings was higher compared to that reached at needle maturity. Repeated removal of single needles (at weekly intervals during growth) from 5-year-old saplings did not influence the composition of monoterpenes. Within a natural Scots pine dominated woodland, 18% of mature Scots pines (N鈥?鈥?74) belonged to the 鈥渘o-螖3-carene鈥?chemotype. Chemotypic variation within populations means that the statistical power with which differences in monoterpene concentrations can be detected is lower when sampling from the whole population compared to sampling within chemotypes. Reduction of this background variation and accounting for chiral variation if present, would significantly aid efficiency, interpretation, and understanding of processes in chemical and ecological research. One method for achieving this is the screening of plants for chemotypes before the establishment of experiments or field sampling regimes. We present a summary of suitable analytical methods for needle tissue that facilitates this prior screening.