Differential pulse voltammetric assay for the carcinoembryonic antigen using a glassy carbon electrode modified with layered molybdenum selenide, graphene, and gold nanoparticles

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• 作者：Baoshan He
• 关键词：Two ; dimension transition metal dichalcogenides ; Ultrasensitive detection ; Tumor marker ; Aptamer ; Electrochemical sensor ; Gold ; thiol chemistry
• 刊名：Microchimica Acta
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：184
• 期：1
• 页码：229-235
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering;
• 出版者：Springer Vienna
• ISSN：1436-5073
• 卷排序：184

文摘

A highly sensitive electrochemical aptasensor is described for the determination of carcinoembryonic antigen (CEA). It is based on the use of a hybrid material composed of molybdenum selenide, graphene, and gold nanoparticles (AuNPs). The MoSe₂-graphene hybrid was prepared by a hydrothermal method and used as the supporting substrate. It was placed on the surface of a glassy carbon electrode onto which the AuNPs were electrochemically deposited. Thiol-labeled aptamer against CEA was immobilized on the electrode via gold-thiol binding. The use of AuNPs coupled to the MoSe₂-graphene hybrid allows for a large loading with aptamers. Under optimum conditions and at a working potential of 0.21 V (vs. SCE), the assay has a linear calibration plot in the 0.1 pg mL−1 to 100 ng mL−1 CEA concentration range, with a detection limit of 0.03 pg mL−1.